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MAK159

Sigma-Aldrich

Mitochondrial Membrane Potential Kit

sufficient for 500 fluorometric tests (microplate readers)

Sinónimos:

JC-10 Assay, JC-10 Mitochondrial Membrane Potential Assay

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

uso

sufficient for 500 fluorometric tests (microplate readers)

método de detección

fluorometric

enfermedades relevantes

cancer

temp. de almacenamiento

−20°C

Descripción general

Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain.[1] During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.[2][3]

Aplicación

Mitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential.[4][5]

Idoneidad

This kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.

Principio

This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
3511821
3321093
3511821
3321093
3041362

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Na Yang et al.
Biochemical and biophysical research communications, 517(1), 111-117 (2019-07-16)
Doxorubicin (DOX) is a potent anti-neoplastic agent with cumulative cardiotoxicity. DOX-induced cardiotoxicity has been shown to depend on the different dosing times. However, the basis for determining the dosing time to minimize DOX-induced cardiotoxicity and the underlying mechanisms remain incompletely
Giulia Allavena et al.
Cell death & disease, 9(7), 780-780 (2018-07-15)
The cellular recycling pathway of autophagy plays a fundamental role in adaptive responses to nutrient deprivation and other forms of stress under physiological and pathological conditions. However, autophagy can also be a double-edge sword during certain bacterial infections (such as
The mitochondrial membrane potential (deltapsi(m)) in apoptosis.
Jennifer D L, et al.
Apoptosis, 8, 116-128 (2003)
Hemlata et al.
ACS omega, 6(15), 10383-10395 (2021-06-01)
In recent decades, biodegradable polymeric nanoparticles have been used as a nanocarrier for the delivery of anticancer drugs. In the present study, we synthesize bovine serum albumin (BSA) nanospheres and evaluate their ability to incorporate a plant extract with anticancer
Rui Zhang et al.
Brain research, 1652, 135-143 (2016-10-04)
Abnormal gene expression, including mRNAs, and microRNAs (miRNA), have been identified in the development of Alzheimer's disease (AD). Although mitofusin2 (mfn2) has been found to be down-regulated in the neurons from hippocampus and cortex in AD patients, little is known
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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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