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L1668

Sigma-Aldrich

Lectina de Phaseolus vulgaris (frijol rojo)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Sinónimos:

Aglutinina de Phaseolus vulgaris, PHA

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

200

Línea del producto

BioReagent

Formulario

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

potencia

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

técnicas

cell culture | mammalian: suitable

color

white to off-white

solubilidad

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

temp. de almacenamiento

2-8°C

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Aplicación

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for the stimulation of cluster of differentiation (CD4+) T cells[1]
  • for the activation of primary peripheral blood lymphocytes[2]
  • for the stimulation of peripheral blood mononuclear cells[3][4]

Acciones bioquímicas o fisiológicas

La PHA consta de dos especies moleculares, la eritroaglutinina (PHA-E), que tiene poca actividad mitógena y elevada actividad de eritroaglutinina, y la leoucoaglutinina (PHA-L), que tiene elevada actividad mitógena y leoucoaglutinante, pero muy baja actividad eritroaglutininante
La PHA-E no es específica de grupo sanguíneo, pero la aglutinación puede ser inhibida por ciertos oligosacáridos. La PHA-P es la forma proteica de la PHA antes de la separación y la purificación de la eritroaglutinina y la leoucoaglutinina. La PHA-M es la forma mucoproteínica. Los conjugados se preparan a partir de las correspondientes lectinas purificadas.

Envase

Sold on the basis of lectin content

Nota de análisis

Potency and solubility expressed in mg lectin/ml

Producto relacionado

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000408863
0000406766
0000384022
0000354443
SLCM5481

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Marcell Costa de Medeiros et al.
Oncotarget, 8(37), 60860-60874 (2017-10-06)
The immune system detects shifts from homeostasis and eliminates altered cells. However, neoplastic cells can modulate the host response to escape immunosurveillance thereby allowing tumor progression. Head and neck squamous cell carcinoma (HNSCC) is one of the most immunosuppressive cancers
Posttranscriptional Regulation of HIV-1 Gene Expression during Replication and Reactivation from Latency by Nuclear Matrix Protein MATR3
Sarracino A, et al.
mBio, 9(6), e02158-e02118 (2018)
Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression
Wang M, et al.
Retrovirology, 15(1), 65-65 (2018)
Nina Thakkar et al.
Antimicrobial agents and chemotherapy, 53(2), 631-638 (2008-12-03)
The present studies were conducted to better define the mechanism of action of polyethylene hexamethylene biguanide (PEHMB) (designated herein as NB325), which was shown in previous studies to inhibit infection by the human immunodeficiency virus type 1 (HIV-1). Fluorescence-activated flow
Ema T Crooks et al.
PLoS pathogens, 14(5), e1007024-e1007024 (2018-05-03)
The extensive glycosylation of HIV-1 envelope (Env) glycoprotein leaves few glycan-free holes large enough to admit broadly neutralizing antibodies (bnAb). Consequently, most bnAbs must inevitably make some glycan contacts and avoid clashes with others. To investigate how Env glycan maturation
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Questions

1–7 of 7 Questions  

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Helpful?

  2. How should the PHA solution using Phytohemagglutinin PHA-P powder be prepared for cell culture?

    1 answer

    1. The stock solution of this product can be prepared in phosphate buffered saline at pH 6.8, resulting in a clear to slightly hazy solution at a concentration of 5 mg per mL. This stock solution can then be diluted in cell culture medium to a working concentration of 10 micrograms of lectin per mL for use in peripheral blood cell culture.

      Helpful?

  3. What is the shelf life once reconstituted in PBS?

    1 answer

    1. After reconstitution in PBS, this product can be stored at 2-8°C for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions of at least 1 mg/mL can be stored in frozen aliquots. Do not dilute to the working concentration until ready to use. Vortex stock solutions prior to use.

      Helpful?

  4. What is the solution stability for Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer

    1. The solution stability was not evaluated by Sigma-Aldrich; however, references indicate media containing dilute PHA can be stored refrigerated for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions can be stored in frozen aliquots.

      Helpful?

  5. How can Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder, be dissolved?

    1 answer

    1. Product No. L1668 is solubility tested using 5 mg per mL of phosphate buffered saline (PBS), pH 6.8; this yields a clear to slightly hazy, colorless to light yellow solution.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What is the mitogenic activity of Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer

    1. The minimum specification for mitogenic activity of this product is  < or = 10 micrograms lectin/mL of medium. The reported specification is PASS. A specific value is not given.

      Helpful?

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