If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
D8417
4′,6-Diamidino-2-phenylindole dihydrochloride
powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence
Sinónimos:
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
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| Talla/SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
product line
BioReagent
Quality Segment
assay
≥98% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 20 mg/mL, PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O
fluorescence
λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
General description
Application
Biochem/physiol Actions
Disclaimer
1 of 1
Este artículo | |||
|---|---|---|---|
| description powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence | description suitable for fluorescence, BioReagent, ≥95.0% (HPLC) | description For Nuclear counterstain in Fluorescence Microscopy | description 4′,6-Diamidine-2′-phenylindole dihydrochloride |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) DNA sequencing: suitable, DNA extraction: suitable, PCR: suitable | technique(s) - |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for nucleic acid staining | suitability - |
| fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA, λex 340 nm; λem 488 nm (nur DAPI) | fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI) | fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI) |
| form powder | form powder | form liquid | form powder |
| solubility H2O: 20 mg/mL, PBS: insoluble | solubility DMF: soluble, H2O: soluble | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C | storage temp. room temp |
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Palabra de advertencia
Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Órganos diana
Respiratory system
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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