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Sigma-Aldrich

4′,6-Diamidino-2-phenylindole dihydrochloride

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

Sinónimos:

2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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About This Item

Fórmula empírica (notación de Hill):
C16H15N5 · 2HCl
Número de CAS:
28718-90-3
Peso molecular:
350.25
Beilstein:
4894417
Número CE:
249-186-7
Número MDL:
MFCD00012681
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
57648699
NACRES:
NA.32

Línea del producto

BioReagent

Ensayo

≥95.0% (HPLC)

Formulario

powder

solubilidad

DMF: soluble
H2O: soluble

fluorescencia

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

idoneidad

suitable for fluorescence

temp. de almacenamiento

room temp

cadena SMILES

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

Clave InChI

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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Aplicación

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Acciones bioquímicas o fisiológicas

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Envase

Bottomless glass bottle. Contents are inside inserted fused cone.

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H315,H317,H335

Clasificaciones de peligro

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCF5587
BCBZ8675
BCBS7582V
BCBP8312V
BCBL9207V

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Silvia Pivetti et al.
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Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inhibition of non-lineage-specific
Georgia Pennarossa et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(22), 8948-8953 (2013-05-23)
The differentiated state of mature cells of adult organisms is achieved and maintained through the epigenetic regulation of gene expression, which consists of several mechanisms including DNA methylation. The advent of induced pluripotent stem cell technology enabled the conversion of
Carlos Humberto Andrade-Moraes et al.
Brain : a journal of neurology, 136(Pt 12), 3738-3752 (2013-10-19)
Alzheimer's disease is the commonest cause of dementia in the elderly, but its pathological determinants are still debated. Amyloid-β plaques and neurofibrillary tangles have been implicated either directly as disruptors of neural function, or indirectly by precipitating neuronal death and
Ruth Halaban et al.
PloS one, 4(2), e4563-e4563 (2009-02-24)
Decitabine, an epigenetic modifier that reactivates genes otherwise suppressed by DNA promoter methylation, is effective for some, but not all cancer patients, especially those with solid tumors. It is commonly recognized that to overcome resistance and improve outcome, treatment should
Shriparna Sarbajna et al.
Genes & development, 28(10), 1124-1136 (2014-05-17)
The resolution of recombination intermediates containing Holliday junctions (HJs) is critical for genome maintenance and proper chromosome segregation. Three pathways for HJ processing exist in human cells and involve the following enzymes/complexes: BLM-TopoIIIα-RMI1-RMI2 (BTR complex), SLX1-SLX4-MUS81-EME1 (SLX-MUS complex), and GEN1.
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