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A1543

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

Goat Anti-Human IgG (whole molecule)−AP

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About This Item

Número MDL:
MFCD00162453
Código UNSPSC:
12352203
NACRES:
NA.46
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

goat

recombinante

expressed in goat

conjugado

alkaline phosphatase conjugate

forma del anticuerpo

IgG fraction of antiserum

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

técnicas

direct ELISA: 1:20,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains, with molecular weight of 50kDa and 25kDa respectively. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG binds to human IgG. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains.

Inmunógeno

IgG purified from human serum

Aplicación

Goat anti-human IgG can also be used for dot blot (1:30,000) and immunohistochemical (1:50) applications.
Serum human IgG levels were analyzed by solid phase ELISA assays using alkaline phosphatase-conjugated goat anti-human IgG antibody incubated for 1 hour at 37°C. Assays was developed using 0.1% p-nitrophenyl phosphate (Sigma) as the substrate. Antibodies to the Seoul (strain 80/39) virus was detected in human sera by western blot analysis using alkaline phosphatase-conjugated goat anti-human IgG antibody at a 1:1000 dilution. Blots were developed using nitroblue tetrazolium and 5-bromo-4-chloro-3-indoyl-phosphate substrate (Sigma).

Forma física

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000341554
0000328714
0000329427
0000245307
0000152023

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Jens Bukh et al.
Journal of medical virology, 80(1), 87-94 (2007-11-28)
GB virus-B (GBV-B) is the virus most closely related to hepatitis C virus (HCV). Thus, we have used GBV-B infection of tamarins, which develop acute hepatitis following experimental infection, as a surrogate model to study protective immunity. As challenge virus
L Lledó et al.
Journal of medical microbiology, 51(10), 861-865 (2002-11-19)
Data relating to hantavirus infection in Spain are scarce and limited to rural areas. The aim of this work was to study the seroprevalence of hantavirus infection in the Autonomous Community of Madrid (ACM), a region containing both rural and
Jessica E Manning et al.
The Journal of infectious diseases, 226(8), 1327-1337 (2021-11-01)
We established the first prospective cohort to understand how infection with dengue virus is influenced by vector-specific determinants such as humoral immunity to Aedes aegypti salivary proteins. Children aged 2-9 years were enrolled in the PAGODAS (Pediatric Assessment Group of
Heetae Lee et al.
Journal of medical virology, 84(12), 1943-1952 (2012-10-20)
A highly significant increase in anti-Vesivirus (family Caliciviridae) antibody prevalence, along the axis from healthy blood donors; donors with elevated transaminase; patients with clinical hepatitis; and patients with post-transfusion/dialysis hepatitis, has been reported in human sera from the USA and
Sophana Chea et al.
Frontiers in immunology, 15, 1368066-1368066 (2024-05-16)
Aedes spp. are the most prolific mosquito vectors in the world. Found on every continent, they can effectively transmit various arboviruses, including the dengue virus which continues to cause outbreaks worldwide and is spreading into previously non-endemic areas. The lack
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Questions

1–3 of 3 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  2. What is the source of the alkaline phosphatase that is used to make Product A1543, Anti-Human IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat?

    1 answer

    1. Product A1543, Anti-Human IgG (whole molecule) -Alkaline Phosphatase antibody produced in goat, is prepared using alkaline phosphatase from bovine intestinal mucosa.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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