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Merck

A3150

Sigma-Aldrich

Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Sinónimos:

Goat Anti-Human IgG (γ-chain specific)−AP

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

alkaline phosphatase conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous glycerol solution

reactividad de especies

human

técnicas

direct ELISA: 1:7,000-1:21,000

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

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Descripción general

Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.

Inmunógeno

purified human IgG

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody can be used for ELISA applications using serum samples from allergic and borderline hypertensive patients.
Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. Membranes were developed using BCIP/NBT substrate (Sigma).
Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).

Acciones bioquímicas o fisiológicas

Human IgGs regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones Kappa, and Lambda myeloma proteins.
IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions. IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region aids in the development of therapeutic antibody.

Forma física

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2


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P Hühn et al.
Nucleic acids research, 25(2), 410-416 (1997-01-15)
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La
X Baur et al.
Allergy, 57(10), 943-945 (2002-09-25)
Although fungal phytase is frequently used as an additive to animal feed few investigations of its allergenic property have been conducted. Fifty-three subjects occupationally exposed to powdered phytase from Aspergillus niger were studied. Exposure data and symptoms were registered by
K P Ng et al.
Arthritis and rheumatism, 55(6), 900-904 (2006-12-02)
To identify the prevalence of serologically active clinically quiescent (SACQ) patients in a cohort of 290 patients with systemic lupus erythematosus (SLE). We investigated if the presence of anti-double-stranded DNA (anti-dsDNA) or antinucleosome (anti-NCS) antibodies during the SACQ period was
R Wu et al.
Hypertension (Dallas, Tex. : 1979), 33(1), 53-59 (1999-02-04)
-Elevated antibody levels to oxidized low-density lipoprotein (aOxLDL) have been shown to correlate with the degree of atherosclerosis in some studies. On the other hand, immunization of experimental animals with OxLDL, leading to enhanced aOxLDL levels, inhibits the development of
H Tröster et al.
Journal of autoimmunity, 8(6), 825-842 (1995-12-01)
Immunization of Balb/c mice with a homogeneously purified recombinant human La/SS-B protein resulted in activation of an autoreactive B cell secreting a novel monoclonal anti-La antibody termed La4B6. La4B6 reacted with La protein from a variety of sources including human

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