MABT400
Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody
clone 5C11, from mouse
Sinónimos:
Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
5C11, monoclonal
reactividad de especies
canine, bovine, goat, rat, porcine, human, guinea pig
técnicas
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PRG4(10216)
Descripción general
The protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.
Inmunógeno
Recombinant protein corresponding to human Lubricin/Proteoglycan 4.
Aplicación
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).
This Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.
Calidad
Evaluated by Western Blotting in Lubercin purified protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.
Descripción de destino
~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.
Forma física
Format: Purified
Otras notas
Concentration: Please refer to lot specific datasheet.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Patrick Weber et al.
Advanced healthcare materials, e2401623-e2401623 (2024-07-15)
Osteoarthritis is a degenerative joint disease that is associated with decreased synovial fluid viscosity and increased cartilage friction. Though viscosupplements are available for decades, their clinical efficacy is limited and there is ample need for more effective joint lubricants. This
Dongrim Seol et al.
Human gene therapy, 33(9-10), 529-540 (2021-10-07)
Lubricin, a glycoprotein encoded by the proteoglycan 4 (PRG4) gene, is an essential boundary lubricant that reduces friction between articular cartilage surfaces. The loss of lubricin subsequent to joint injury plays a role in the pathogenesis of posttraumatic osteoarthritis. In
A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity.
Knight-Schrijver, et al.
Nature cardiovascular research, 1, 1215-1229 (2023)
Taryn E Ludwig et al.
BMC musculoskeletal disorders, 16, 386-386 (2015-12-17)
Proteoglycan 4 (PRG4) and hyaluronan (HA) are key synovial fluid constituents that contribute synergistically to cartilage boundary lubrication; however, the effects of their concentrations as well as their structure, both of which can be altered in osteoarthritis, on this functional
Joseph A M Steele et al.
Biomaterials, 286, 121548-121548 (2022-05-20)
Articular cartilage is comprised of zones that vary in architecture, extracellular matrix composition, and mechanical properties. Here, we designed and engineered a porous zonal microstructured scaffold from a single biocompatible polymer (poly [ϵ-caprolactone]) using multiple fabrication strategies: electrospinning, spherical porogen
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