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D5523

Sigma-Aldrich

Medio de Eagle modificado de Dulbecco, poca glucosa

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

DME, DMEM

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About This Item

Número MDL:
MFCD00217342
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

500

Formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Aplicación

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human [1][2] and murine mesenchymal stem cells (MSCs).[3]

Cantidad

Formulated to contain 10.0 grams of powder per liter of medium.

Reconstitución

Supplement with 3.7 g/L sodium bicarbonate.

suplemento

Referencia del producto
Descripción
Precios

también adquirido normalmente con este producto

Referencia del producto
Descripción
Precios

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317,H319

Clasificaciones de peligro

Eye Irrit. 2 - Skin Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000353335
0000312565
0000312452
0000291143
0000311990

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Daniela Steffens et al.
Journal of biomaterials applications, 32(5), 663-676 (2017-10-11)
Laminin-functionalized poly-d,l-lactic acid scaffolds were produced. Following this, mesenchymal stem cells and keratinocytes were seeded on biomaterials for the in vivo experiments, where the biomaterials with or without cells were implanted. The analysis is comprised of the visual aspect and mean
Hsi-Hsien Hsu et al.
Journal of cellular physiology, 233(7), 5458-5467 (2017-12-17)
Oxaliplatin (OXA), is a third generation platinum drug used as first-line chemotherapy in colorectal cancer (CRC). Cancer cells acquires resistance to anti-cancer drug and develops resistance. ATP-binding cassette (ABC) drug transporter ABCG2, one of multidrug resistance (MDR) protein which can
Kallyne K Mimura et al.
Molecular vision, 18, 1583-1593 (2012-06-29)
The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. C57BL/6 female mice were infected using intravitreal injections of either 10(6) tachyzoites of
Ming-Cheng Chen et al.
Environmental toxicology, 33(5), 587-593 (2018-02-13)
Colorectal cancer (CRC) is one of the most common cancers and causes of cancer-related death. There are several first-line chemotherapeutic drugs used to treat CRC. Oxaliplatin (OXA) is an alkylating cytotoxic agent that is usually combined with other chemotherapeutic drugs
Akon Higuchi et al.
Scientific reports, 5, 10217-10217 (2015-05-15)
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs
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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Questions

1–6 of 6 Questions  

  1. How do I prepare Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?

    1 answer

    1. Measure out 90% of final required volume of water. Water temperature should be 15-20 °C.While gently stirring the water, add the powdered medium. Stir until dissolved. Do NOT heat.Rinse original package with a small amount of water to remove all traces of powder. Add to solution in step 2.To the solution in step 3, add 3.7 g sodium bicarbonate or 49.3 ml of sodium bicarbonate solution [7.5%w/v] for each liter of final volume of medium being prepared. Stir until dissolved.While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may rise during filtration. The use of 1N HCl or 1N NaOH is recommended.Add additional water to bring the solution to final volume.Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.Aseptically dispense medium into sterile container.

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  2. What sodium bicarbonate should I use with Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?

    1 answer

    1. Sodium Bicarbonate powder (Product No. S5761) or Sodium Bicarbonate Solution, 7.5% (Product No. S8761) is recommended.  For more information, consult the product datasheet.

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  3. Do you offer a liquid version of Dulbecco’s Modified Eagle’s Medium - low glucose?

    1 answer

    1. Product No. D6046 is the fully supplemented liquid form of the original Dulbecco's formulation, offered as Product No. D5523, with one key exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.  D5546 is the same but does not contain L-glutamine.

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  4. What is the formulation of Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?

    1 answer

    1. The formulation can be found on the DMEM formulation sheet at the following link.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. How do I store Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose, dry powder?  How do I store the reconstituted medium?

    1 answer

    1. Store the dry powdered medium at 2-8 °C under dry conditions and liquid medium at 2-8 °C in the dark.  The nature of supplements added may affect storage conditions and shelf life of the medium. The product label bears the lot-specific expiration date.

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