12-500
HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer
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About This Item
UNSPSC Code:
41105330
eCl@ss:
32011807
NACRES:
NA.42
Productos recomendados
biological source
human
Quality Level
form
liquid
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
shipped in
dry ice
General description
EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)
Cell Line Description
HeLa
Physical form
100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.
Storage and Stability
Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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D V Marinkovic et al.
The Biochemical journal, 165(3), 417-423 (1977-09-01)
Carboxymethylated beta-galactosidase from Escherichia coli was dissociated at 100 degrees C to form carboxymethylated fragments A and B. The mol.wts. of carboxymethylated fragments A and B were determined by gel filtration to be 64300 and 22400 respectively. Sodium dodecyl sulphate/polyacrylamide-gel
D V Marinkovic et al.
The Biochemical journal, 155(2), 209-216 (1976-05-01)
Aminoethylated beta-galactosidase from Escherichia coli was cleaved by CNBr. The fragment C4a was purified by gel filtration and ion-exchange chromatography. The molecular weight of the fragment C4a was determined to be 9000 +/- 600. The N-terminal amino acid was found
Brandon S Carpenter et al.
Journal of cell science, 128(5), 1034-1050 (2015-01-16)
GLI transport to the primary cilium and nucleus is required for proper Hedgehog (HH) signaling; however, the mechanisms that mediate these trafficking events are poorly understood. Kinesin-2 motor proteins regulate ciliary transport of cargo, yet their role in GLI protein
G De Ritis et al.
Pediatric research, 13(11), 1255-1261 (1979-11-01)
A peptic-tryptic-cotazym (PTC) digest of a crude wheat gliadin preparation was obtained under experimental conditions simulating in vivo protein digestion and then fractionated into 10 peaks by ion-exchange chromatography. PTC-gliadin digest and one of its subfractions (coded as fraction 9
Ioannis Partheniadis et al.
Pharmaceutics, 12(6) (2020-06-25)
Oregano essential oil (EO) enteric release powder was formulated by spray drying feed emulsions stabilized with polysaccharides (PSC) and Eudragit® L100 (PLM). Different modified starches were used in the PSC component. Spray-dried powders were evaluated for particle size and morphology
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