T7539

Tumor Necrosis Factor-α from mouse

≥95%, recombinant, expressed in E. coli, powder, suitable for cell culture

• Sinónimos: mTNF-α, TNF-α
• Número MDL: MFCD00164823
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Nombre del producto: Tumor Necrosis Factor-α from mouse, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Ensayo: ≥95%
• Formulario: powder
• potencia: 0.01-0.5 ng/mL EC₅₀
• mol peso: 17 kDa
• envase: pkg of 10 μg; pkg of 50 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• impurezas: ≤1 EU/μg protein endotoxin
• color: white
• solubilidad: water: soluble
• Nº de acceso UniProt: P06804
• temp. de almacenamiento: −20°C
• Información sobre el gen: mouse ... Tnf(21926)

Descripción

Aplicación

Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:

• to activate MS1 endothelium cells[1]
• to induce lipolysis in adipocytes[2]
• to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation[3]

Acciones bioquímicas o fisiológicas

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.

Tumor necrosis factor-α, also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.

Definición de unidad

One unit is the amount required to induce half-maximal cytolysis of L929 cells.

Forma física

Lyophilized from 10 mM sodium phosphate, pH 7.5

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H315,H319
• Consejos de prudencia: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Skin Irrit. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)