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T6674
Tumor Necrosis Factor-α human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture
Sinónimos:
hTNF-α, TNF-α
About This Item
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Nombre del producto
Tumor Necrosis Factor-α human, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
origen biológico
human
Nivel de calidad
recombinante
expressed in E. coli
Ensayo
≥97% (SDS-PAGE)
Formulario
powder
potencia
0.02-0.3 ng/mL ED50/EC50
calidad
endotoxin tested
mol peso
~17.4 kDa
envase
pkg of 5x10 μg
pkg of 10 μg
condiciones de almacenamiento
avoid repeated freeze/thaw cycles
técnicas
cell culture | mammalian: suitable
impurezas
<1 EU/μg
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Información sobre el gen
human ... TNF(7124)
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Aplicación
- to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells[1]
- to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression[2]
- to induce death-receptor-mediated apoptosis in HeLa cells[3].
Acciones bioquímicas o fisiológicas
Forma física
Nota de análisis
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What is the Department of Transportation shipping information for this product?
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What is the solubility of this Product T6674, Tumor Necrosis Factor-α human?
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This can be dissolved at 0.1 to 1.0 mg/ml in saline or buffer, stabilized with 0.1 to 1%BSA.
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What are the units/mg of Product T6674, Tumor Necrosis Factor-α human?
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1ug=1.1x10e5 units.
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What is the EC50 of Product T6674, Tumor Necrosis Factor-α human?
1 answer-
Bioactivity(cell inhibition, L929 cells) is expressed by an EC50 of 0.02 to 0.05 ng/mL for T6674. EC50 (equivalent concentration) is the same as ED50 (equivalent dose, cytotoxicity, L929 cells) used for the bioactivity specification for T0157, Tumor Necrosis Factor-alpha, human recombinant expressed in Yeast.
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