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Merck

T6674

Sigma-Aldrich

Tumor Necrosis Factor-α human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

Sinónimos:

hTNF-α, TNF-α

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77
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Nombre del producto

Tumor Necrosis Factor-α human, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

origen biológico

human

Nivel de calidad

recombinante

expressed in E. coli

Ensayo

≥97% (SDS-PAGE)

Formulario

powder

potencia

0.02-0.3 ng/mL ED50/EC50

calidad

endotoxin tested

mol peso

~17.4 kDa

envase

pkg of 5x10 μg
pkg of 10 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

técnicas

cell culture | mammalian: suitable

impurezas

<1 EU/μg

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Información sobre el gen

human ... TNF(7124)

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Aplicación

TNF-α include stimulating growth of human fibroblasts and other cell lines activating polymorphonuclear neutrophils and osteoclasts and inducing interleukin-1, prostaglandin E2, and collagenase production.
Tumor Necrosis Factor-α (TNFα) human has been used:
  • to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells[1]
  • to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression[2]
  • to induce death-receptor-mediated apoptosis in HeLa cells[3].

Acciones bioquímicas o fisiológicas

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in anti-tumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.
Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.

Forma física

Lyophilized from a 0.2 μm-filtered buffer solution containing 3mM Tris-HCl and 5% trehalose.

Nota de análisis

The biological activity of TNF-α is measured by the cytolysis of murine L929 cells.

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Certificados de análisis (COA)

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Visite la Librería de documentos

A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation
Skaletskaya A, et al.
Proceedings of the National Academy of Sciences of the USA, 98(14), 7829-7834 (2001)
Role of NonO-histone interaction in TNFalpha-suppressed Prolyl-4-hydroxylase alpha1
Zhang C, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1783(8), 1517-1528 (2008)
miRNA-155 up-regulation and complement factor H (CFH) deficits in Down?s Syndrome
Li YY, et al.
Neuroreport, 23(3), 168-168 (2012)
Casper Steenholdt et al.
The American journal of gastroenterology, 109(7), 1055-1064 (2014-05-07)
Cost-effective guidance of therapeutic strategy in Crohn's disease patients with secondary infliximab (IFX) treatment failure may be achieved by serum IFX and anti-IFX antibody (Ab) measurements by radioimmunoassay (RIA). This study investigated implications of using other techniques for this purpose.
Fen Li et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 40(9), 945-954 (2015-09-27)
To investigate the association of TNF-α-308 gene polymorphism with the risk of RA in Hunan's Han Chinese population. A total of 112 patients with RA from Han Chinese population in Hunan were recruited, along with 129 healthy controls. TNF-α-308 (rs1800629)

Protocolos

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

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Questions

1–4 of 4 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. What is the solubility of this Product T6674, Tumor Necrosis Factor-α human?

    1 answer
    1. This can be dissolved at 0.1 to 1.0 mg/ml in saline or buffer, stabilized with 0.1 to 1%BSA.

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  3. What are the units/mg of Product T6674, Tumor Necrosis Factor-α human?

    1 answer
    1. 1ug=1.1x10e5 units.

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  4. What is the EC50 of Product T6674, Tumor Necrosis Factor-α human?

    1 answer
    1. Bioactivity(cell inhibition, L929 cells) is expressed by an EC50 of 0.02 to 0.05 ng/mL for T6674.  EC50 (equivalent concentration) is the same as ED50 (equivalent dose, cytotoxicity, L929 cells) used for the bioactivity specification for T0157, Tumor Necrosis Factor-alpha, human recombinant expressed in Yeast.

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