The reported molecular weight is between 10-15 million Daltons before activation (nicking), primarily as double-stranded DNA. A double-stranded DNA of 1 kb in length has a molecular weight of 660 kiloDalton. A size of 15 million daltons is equivalent to 15,000 kilodaltons. When multiplied by (1kb/660 kilodaltons), it equals 22.72 kb on average. It's important to note that the average number of base pairs is not measured, so this calculation provides an estimate.
D4522
Deoxyribonucleic acid from calf thymus
Type XV, Activated, lyophilized powder
Sinónimos:
Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
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General description
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Features and Benefits
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Quantity
One mg DNA is approx. 20 A260 units.
Preparation Note
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.[3]
Reconstitution
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
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Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Could you provide the number of base pairs for the D4522 product? For example, is it around 20kb?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product D4522, Deoxyribonucleic acid?
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The molecular weight is reported to be between 10-15 million Daltons prior to activation (nicking).
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Is BSA (bovine serum albumin) used during the method of preparation for Product D4522, Deoxyribonucleic acid?
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During the method of preparation, when deoxyribonucleic acid is nicked, BSA is added to the solution. This is followed by an extraction method to remove all exogenous protein. We do not, however, claim this deoxyribonucleic acid is 100% clean of all proteins (including BSA).
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What is the solution stability of Product D4522, Deoxyribonucleic acid?
1 answer-
Solutions of deoxyribonucleic acid have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (25 μg/mL), DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store deoxyribonucleic acid in alkaline solutions since it tends to degrade at pH greater than 8.0.
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Is Product D4522, Deoxyribonucleic acid, single or double stranded?
1 answer-
Even though it is activated or "nicked" DNA, we have found that it is primarily double stranded.
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