Saltar al contenido
Merck
Todas las fotos(5)

Key Documents

View All Documentation

H7033

Sigma-Aldrich

PerfectHyb™ Plus Hybridization Buffer

for Northern and Southern blotting, solution

Sinónimos:

hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(5)

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

General description

Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.

Application

Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Features and Benefits

  • Fast and easy-to-use
  • Up to 20x more sensitive than conventional buffers
  • Doesn′t require blocking steps
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays
  • Compatible with radioactive and non-radioactive probes
  • Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

Other Notes

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.

Legal Information

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Replaced by

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Yinping Jiao et al.
BMC plant biology, 11, 141-141 (2011-10-22)
miRNAs are known to play important regulatory roles throughout plant development. Until recently, nearly all the miRNAs in maize were identified by comparative analysis to miRNAs sequences of other plant species, such as rice and Arabidopsis. To find new miRNA
Alavala Matta Reddy et al.
BMC genomics, 10, 65-65 (2009-02-07)
MicroRNAs (miRNAs) are small ~22-nt regulatory RNAs that can silence target genes, by blocking their protein production or degrading the mRNAs. Pig is an important animal in the agriculture industry because of its utility in the meat production. Besides, pig
Angel Emilio Martínez de Alba et al.
Nucleic acids research, 39(21), 9339-9344 (2011-08-05)
In plants, most microRNAs (miRNAs) and several endogenous small interfering RNAs (siRNAs) bind to ARGONAUTE1 (AGO1) to regulate the expression of endogenous genes through post-transcriptional gene silencing (PTGS). AGO1 also participates in a siRNA-mediated PTGS defense response that thwarts exogenous
Sivaramakrishnan Muthuswamy et al.
Planta, 233(1), 201-208 (2010-09-28)
Protein sumoylation plays an important role in plant development, flowering-time regulation, and abiotic stress response. However, the molecular role of sumoylation in these pathways is largely unknown. It was shown previously that in mutants of the inner nuclear basket nucleoporin
Edyta Koscianska et al.
TheScientificWorldJournal, 11, 102-117 (2011-01-11)
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists
Ver todos los artículos relacionados

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico