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31149

Sigma-Aldrich

Deoxyribonucleic acid, low molecular weight from salmon sperm

Sinónimos:

Salmon sperm dsDNA, low molecular weight DNA, DNA

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About This Item

Número de CAS:
100403-24-5
EC Number:
309-566-6
MDL number:
MFCD00130921
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grade

for molecular biology
puriss. p.a.

form

lyophilized powder

impurities

≤5% protein

solubility

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

absorbance ratio

A260/280 nm ~1.4

storage temp.

2-8°C

Application

Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
  • to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.[1]
  • in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.[2]
  • as a precursor in the synthesis of the bio-dots.[3]

Biochem/physiol Actions

Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.

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Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCK8483
BCCM3251
BCCL1729
BCCJ7082
BCCH5235

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J J Gao et al.
Journal of immunology (Baltimore, Md. : 1950), 166(11), 6855-6860 (2001-05-22)
LPS is well recognized for its potent capacity to activate mouse macrophages to produce TNF-alpha, an important inflammatory mediator in bacterial infection-related diseases such as septic shock. We demonstrate here that while inducing only low levels of TNF-alpha alone, DNA
Eiji Sunami et al.
Clinical chemistry, 55(3), 559-567 (2009-01-10)
Prostate cancer (PCa) detection using serum-based prostate specific antigen (PSA) is limited by frequent false-positive and -negative results. Genetic aberrations such as allelic imbalance (AI) and epigenetic changes such as promoter hypermethylation have been detected in circulating DNA of cancer
Andrea Hájková et al.
Bioelectrochemistry (Amsterdam, Netherlands), 116, 1-9 (2017-03-18)
A simple electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) onto the GCE surface and subsequently used for the detection of dsDNA damage induced by hydroxyl radicals. Investigation of the mutual
V N Kondratova et al.
Biochemistry. Biokhimiia, 74(11), 1285-1288 (2009-11-18)
A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters
Thiol modification of silicon-substituted hydroxyapatite nanocrystals facilitates fluorescent labelling and visualisation of cellular internalisation
Williams RL et al
Journal of Material Chemistry B: Materials for Biology and Medicine, 35, 4370-4378 (2013)
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Questions

1–2 of 2 Questions  

  1. For the product 31149-50G-F (31149-50G-F), what is the molecular weight, or, the amount of base pairs? Thank you!

    1 answer

    1. The molecular weight of this product is not determined. This material is produced by mechanical disruption, no chemical or enzymatic treatment has been applied. The estimated molecular weight range of this DNA is 50,000 – 100,000 Da and is primarily double stranded. Note that whole DNA from salmon sperm has a molecular weight of 1.98x10e11 Da. While the number of base pairs(bp) has not been investigated, based on a approximate molecular weight of 650 Da per bp, the estimated chain length range is 77 – 154 bp.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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