PANC-1 Cell Line human

87092802, human pancreas, Epithelial

• Sinónimos: PANC1 Cells, Panc-1-P Cells
• Código UNSPSC: 41106514
• eCl@ss: 32190102

Propiedades

• Nombre del producto: PANC-1 Cell Line human, 87092802, from human pancreas
• origen biológico: human pancreas
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, hypertriploid
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian pancreas

Descripción línea celular

Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Aplicación

Human PANC-1 cell line has been used to study the role of the intermediate filament keratin reorganization on the alignment and migration of cancer cells. It has also been used to study calcium-mediated actin reset (CaAR) in response to physiological changes.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8,10
THO1: 7,8
TPOX: 8,11
vWA: 15

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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