This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.
D8537
Disolución salina tamponada con fosfatos de Dulbecco
Dulbecco’s Phosphate Buffered Saline
1 of 6 reviewers received a sample product or took part in a promotion
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Sinónimos:
DPBS
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nombre del producto
Disolución salina tamponada con fosfatos de Dulbecco, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Nivel de calidad
esterilidad
sterile-filtered
Formulario
liquid
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
componentes
glucose: no (Dextro)
sodium pyruvate: no
Condiciones de envío
ambient
¿Está buscando productos similares? Visita Guía de comparación de productos
Descripción general
La disolución salina tamponada con fosfato de Dulbecco (DPBS) es una disolución salina equilibrada (BSS) utilizada para la manipulación y el cultivo de células de mamífero. El tampón DPBS se utiliza para irrigar, lavar y diluir las células de mamífero mientras se mantiene el equilibrio osmótico. Proporciona agua e iones inorgánicos esenciales para el metabolismo celular normal. El DPBS con calcio y magnesio facilita la unión y el agrupamiento celulares. El PBS de Dulbecco sin estos iones puede utilizarse para lavar y enjuagar las células suspendidas. El tampón de fosfato mantiene el pH en el intervalo fisiológico.
Aplicación
La disolución salina tamponada con fosfato de Dulbecco se ha utilizado:
- para la dilución de glóbulos rojos recién extraídos para estudiar la captura del agregado de eritrocitos.[1]
- para lavar los pocillos de una placa de microvaloración.[2]
- para lavar las células antes de resuspenderlas en tampón de lisis para el aislamiento del ADN[3]
- como componente del medio de conservación de embriones para la vitrificación de embriones.
también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
Arturo Levican et al.
Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Cameron Goertzen et al.
Biological procedures online, 20, 20-20 (2018-10-26)
Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main
Gensheng Wang et al.
Molecular therapy. Methods & clinical development, 3, 15052-15052 (2016-01-28)
Interleukin-1 (IL-1) plays an important role in the pathophysiology of osteoarthritis (OA), and gene transfer of IL-1 receptor antagonist (IL-1Ra) holds promise for OA treatment. A preclinical safety and biodistribution study evaluated a self-complementary adeno-associated viral vector carrying rat IL-1Ra
Protocolos
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
-
Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide
1 answer-
Helpful?
-
-
Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?
1 answer-
This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.
Helpful?
-
-
What is the concentration of the product in Molarity?
1 answer-
This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
Product D8537 contains:
Potassium chloride: 0.2 g/l
Potassium phosphate monobasic: 0.2 g/l
Sodium chloride: 8 g/l
Sodium Phosphate dibasic: 1.15 g/lPlease see the link below for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdfHelpful?
-
-
What is the bottle thread type on the 1L bottle (D8537-1L)
1 answer-
The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
Thread Type 38-430
Thread Diameter 37.3 mmHelpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?
1 answer-
This is a 1X concentration. The 10X concentration is listed under product D1408.
Helpful?
-
-
What is a Salt Toxicity Test?
1 answer-
The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.
Helpful?
-
-
What is the formulation for Product D8537, Dulbecco's Phosphate?
1 answer-
The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.
Helpful?
-
-
What is the pH of a solution of Product D8537, Dulbecco's Phosphate?
1 answer-
The pH will be in the range of 7.1-7.5, according to the specification sheet.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
