This product has not been validated for use with the megakaryoblastic cell line DAMI. However, literature indicates compatibility with this cell line. The following citation may be of interest: PLoS One. 2020 Apr 7;15(4):e0230507.
R8758
Medio RPMI-1640
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sinónimos:
Roswell Park Memorial Institute 1640 medium
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Nombre del producto
Medio RPMI-1640, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Nivel de calidad
esterilidad
sterile-filtered
Formulario
liquid
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
componentes
NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
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Descripción general
El medio RPMI 1640 lo desarrolló el equipo de Moore en el Instituto Roswell Park Memorial en 1966. Se formuló una modificación del medio McCoy 5A para sostener las células linfoblastoides en cultivo en suspensión, pero desde entonces se ha demostrado que sirve de soporte para una amplia variedad de células que necesitan anclaje. Originalmente indicado para utilizar con un complemento sérico, se ha demostrado que el RPMI 1640 respalda diversas líneas celulares en ausencia de suero. También se ha utilizado ampliamente en protocolos de fusión y en el crecimiento de células híbridas. Este medio es adecuado para el cultivo de leucocitos humanos sanos y neoplásicos.
Aplicación
Opcional
Referencia del producto
Descripción
Precios
Producto relacionado
también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Good Morning, does RPMI medium R8758 which contains sodium bicarbonate, affect cell suspension culture of megakaryoblastic cell line DAMI? I mean, sodium bicarbonate can significantly modify cell behavior or his effects can be tolerated?
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I bought this product and realized that it does not contain Hepes. Can I add hepes?
1 answer-
This product utilizes a bicarbonate buffering system. However, there are no anticipated problems with the addition of HEPES. We do offer RPMI formulations containing both sodium bicarbonate and HEPES, such as Products R5886 & R7638.
Please see the RPMI formulation tables at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf
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Considering that I have to use an RPMI medium containing 4mM of l-Glutamine and 10mM of non-essential amino acids, can I use the product R8748 without adding anything?
1 answer-
The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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buenos dias, llevo años usando vuestro producto RPMI 1640, y me gustaría saber la composición del medio para ver si hay algun metal/producto que no puede utilizar con un suplemento que quiero introducir. Muchas gracias
1 answer-
Please see the link below to review the RPMI formulation tables. This product is listed in the second column of page 2:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdfHelpful?
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Does Product R8758, RPMI-1640 Medium, contain glucose?
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RPMI contains glucose at 2 g/L. The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.
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Does Product R8758, RPMI-1640 Medium, contain phenol red?
1 answer-
This media contains phenol red. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
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Can Product R8758, RPMI-1640 Medium, be frozen?
1 answer-
We do not recommend freezing cell culture media. A precipitate may form upon thawing. Also, this can cause a loss of amino acids, which may yield low cell growth.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product R8758, RPMI-1640 Medium, contain HEPES?
1 answer-
There is no HEPES in this formulation of RPMI. We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
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Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?
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This formulation of RPMI already contains L-glutamine at 0.3 g/L. It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).
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