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A8111

Sigma-Aldrich

Plasminogen activator inhibitor 1 (PAI-1)

≥200,000 units/mg protein, lyophilized powder, ≥90% (SDS-PAGE)

Synonym(s):

PAIE, PLANH1, Plasminogen activator inhibitor, β-migrating endothelial cell-derived type, Serpin E1 PAI-1, Serpine 1

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32

product name

Plasminogen activator inhibitor 1 (PAI-1) human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

recombinant

expressed in E. coli

Quality Level

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥200,000 units/mg protein

mol wt

~43 kDa

solubility

water: 0.1 mL, clear, colorless

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SERPINE1(5054)

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General description

Plasminogen activator inhibitor 1 (PAI-1) or serine protease inhibitor E1 (SERPINE1) gene is mapped to human chromosome 7q22.1. PAI-1 is produced in hepatocytes, endothelial cells, smooth muscle cells, megakaryocytes, and adipocytes.

Application

Plasminogen activator inhibitor 1 (PAI-1) human has been used:
  • as an inhibitor of pro-brain-derived neurotrophic factor (proBDNF) in radioimmunoprecipitation assay (RIPA) in peripheral blood and lymphocytes
  • to block plasmin activation in mice infected with C. albicans hyphae
  • to test its effect on neurite growth in neurons

Biochem/physiol Actions

Plasminogen Activator Inhibitor 1 (PAI-1) protein belongs to the Serpin superfamily of serine protease inhibitors. It is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and therefore inhibits the fibrinolysis process. PAI is involved in extracellular matrix remodeling and has been implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis. PAI-1 is present in increased levels in various disease states such as a number of cancer forms, obesity and the metabolic syndrome.
Plasminogen activator inhibitor–1 (PAI -1) participates in mediating cellular senescence. It is upregulated during injury along with other tissue repair transcriptomes. Elevated levels of PAI-1 is regarded as one of the risks associated with myocardial infarction (MI).

Unit Definition

1 unit irreversibly inhibits (100% inhibition) 1 unit of tissue plasminogen activator (tPA) under physiological conditions (neutral pH and 37 ºC).

Physical form

Supplied as a lyophilized powder from a solution containing 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Reconstitution

Reconstitute to 0.25 mg/ml with ultrapure water. The reconstituted product contains 0.25 mgP/mL in 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Analysis Note

The product is the active human PAI-1 protein with a molecular weight of 43 kDa.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Liying Lin et al.
Journal of psychiatric research, 133, 166-173 (2020-12-21)
Previous studies showed that blood BDNF levels in mood disorders were reduced. However, little is known about the changes of BDNF and its precursor proBDNF in lymphocytes. In addition, earlier studies using commercial ELISA kits cannot distinguish mature BDNF from
William Santus et al.
Science immunology, 2(15) (2017-09-25)
Nuclear factor of activated T cells (NFAT) is activated in innate immune cells downstream of pattern recognition receptors, but little is known about NFAT's functions in innate immunity compared with adaptive immunity. We show that early activation of NFAT balances
Matteo Cesari et al.
Cardiovascular therapeutics, 28(5), e72-e91 (2010-07-16)
The close relationship existing between aging and thrombosis has growingly been studied in this last decade. The age-related development of a prothrombotic imbalance in the fibrinolysis homeostasis has been hypothesized as the basis of this increased cardiovascular and cerebrovascular risk.
Kirwin M Providence et al.
Archives of dermatological research, 300(6), 303-310 (2008-04-04)
Cutaneous tissue injury, both in vivo and in vitro, initiates activation of a "wound repair" transcriptional program. One such highly induced gene encodes plasminogen activator inhibitor type-1 (PAI-1, SERPINE1). PAI-1-GFP, expressed as a fusion protein under inducible control of +800
Sadiya S Khan et al.
Science advances, 3(11), eaao1617-eaao1617 (2017-11-21)
Plasminogen activator inhibitor-1 (PAI-1) has been shown to be a key component of the senescence-related secretome and a direct mediator of cellular senescence. In murine models of accelerated aging, genetic deficiency and targeted inhibition of PAI-1 protect against aging-like pathology

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