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IP02

Protein A Agarose Suspension

Protein A Agarose Suspension designed for immunoprecipitation applications

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About This Item

NACRES:
NA.56
UNSPSC Code:
41116133
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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG2a and IgG2b and rabbit IgG.
Protein A conjugated to agarose.

Physical form

In PBS.

Other Notes

Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
IP05IP04P1925
Protein A Agarose Suspension Protein A Agarose Suspension designed for immunoprecipitation applications

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IP02

Protein A Agarose Suspension

Protein G Plus/Protein A Agarose Suspension Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Millipore

IP05

Protein G Plus/Protein A Agarose Suspension

Protein G Plus-Agarose Suspension Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Millipore

IP04

Protein G Plus-Agarose Suspension

Protein A–Agarose macrobeads aqueous suspension

Millipore

P1925

Protein A–Agarose macrobeads

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

form

aqueous suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

storage condition

do not freeze

storage condition

do not freeze

storage condition

do not freeze

storage condition

-

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
IP02-1.5ML04055977220209

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