PCR Supplies & Equipment

The polymerase chain reaction (PCR) is a powerful core molecular biology technique. It is an efficient and rapid in vitro method for enzymatic amplification of specific DNA or RNA sequences from nucleic acids of various sources. A simple PCR reaction consists of a set of synthetic oligonucleotide primers that flank the target DNA sequence, target DNA, a thermostable DNA polymerase and dNTPs. A repetitive series of cycles involving template denaturation, primer annealing, followed by extension of the annealed primers, yields tremendous amounts of DNA. Because the strands synthesized in one cycle serve as a template in the next, a million-fold increase in the DNA amount is achieved in just 20 cycles. From PCR microplates, plate covers, pipette tips, PCR tubes, and thermocyclers, explore our comprehensive offering of PCR consumables and equipment to support every step of your PCR workflow. 

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• Products
• PCR thermocyclers and mini cyclers for dna amplification
• PCR plates, strips and films
• PCR pipette tips and filter tips
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PCR Thermocyclers and Mini Cyclers for DNA Amplification

A thermal cycler (also known as a PCR machine or thermocycler) is a laboratory instrument that heats and cools samples in repetitive cycles to facilitate DNA or RNA amplification through the polymerase chain reaction. These machines have become commonplace in labs, although the introduction of mini or portable thermal cyclers has made it easier to perform PCR reactions in the field and classroom. Many thermal cyclers are programmable and allow users to save frequently used protocols. PCR typically requires 20 to 35 cycles comprising two to three temperature steps (denaturation, annealing, and extension). Combining speed, flexibility, and PCR optimization, the Eppendorf^® Mastercycler Thermocyclers are suitable for enhanced PCR functions, like the 2D-Gradient and thorough documentation capabilities. Additionally, we also offer Benchmark Scientific TC models of thermocyclers that are designed for lower throughput needs, while maintaining the same functionality and a lower price point compared to larger units. 

Additionally, discover a variety of additional PCR plate seals, including eXTReme™ plate seals, ThermalSeal RTS™ plate seals, and AlumaSeal^® PCR plate seals to accommodate your PCR workflow needs.