GF144
Epidermal Growth Factor Protein, Human recombinant
The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.
Synonym(s):
EGF
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
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Quality Level
Assay
>98% (SDS-PAGE)
specific activity
≥1 x 10^7 U/mg
manufacturer/tradename
Chemicon®
impurities
<0.1 ng/μg Endotoxin (of EGF; 1EU/μg)
input
sample type neural stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Product Source: E.coli
Application
For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
Linkage
Replaces: GF001
Physical form
Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.
Storage and Stability
The lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.
Analysis Note
Specific Activity: Human EGF is fully biologically active when compared to standards.The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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Protocols
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
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