The limit of detection for Product No. T0440, the 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System used in ELISA, is specified to range from 3 to 6 pg/ml.
T0440
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Synonym(s):
TMB
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100 ML
€211.00
1 L
€1,560.00
5 X 1 L
€3,530.00
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100 ML
€211.00
1 L
€1,560.00
5 X 1 L
€3,530.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Recommended Products
Product Name
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate
Quality Level
form
liquid
storage temp.
2-8°C
General description
TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP.
Application
TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.
How to use our TMB substrate:
How to use our TMB substrate:
- Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
- Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
- Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
- 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System is not recommended for membrane applications. Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
recommended
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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What is the limit of detection for Product No. T0440, the 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System used in ELISA?
1 answer-
Helpful?
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What is the concentration of the TMB and what is the solvent in Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
1 answer-
The concentration of TMB in this formulation and the identity of the solvent are considered proprietary information. The product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer.
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Do I need to dilute Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
1 answer-
No, the product should not be diluted.. The product is ready-to-use for ELISA applications. If the intensity of the reaction needs to be reduced, it is recommended that the antibodies or conjugates be diluted. Usage information is described on our data sheet.
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What is the difference between products T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, and T8665, 3,3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System?
1 answer-
Both products are comparable and either one can be used in ELISA procedures. The formulations which are proprietary may have some slight differences.
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Do I need to add hydrogen peroxide to Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
1 answer-
No hydrogen peroxide should be added to product T0440. The product, which contains a peroxidase substrate, already contains hydrogen peroxide. This solution is ready to use for ELISA assays.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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