T6424

Trypsin from human pancreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

• Sinónimos: Serine Protease 1
• Número de CAS: 9002-07-7
• Comisión internacional de enzimas: 3.4.21.4 (BRENDA, IUBMB)
• EC Number: 232-650-8
• MDL number: MFCD01323069
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: human pancreas
• Quality Level: 200
• form: salt-free, lyophilized powder
• packaging: vial of ≥1,000 BAEE units
• UniProt accession no.: P07477; P07478; P35030
• storage temp.: −20°C
• Gene Information: human ... PRSS1(5644) , PRSS2(5645) , PRSS3(5646)

Descripción

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.[1]

Application

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. [2] Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells. [3]

Unit Definition

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Quantity

Minimum 1,000 BAEE units/vial

Analysis Note

Protein determined by E1%/280.

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves