P0157

Phosphatase, Acid from potato

ammonium sulfate suspension, ≥200 units/mg protein (biuret)

• Sinónimos: (Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
• Número de CAS: 9001-77-8
• Comisión internacional de enzimas: 3.1.3.2 (BRENDA, IUBMB)
• Número CE: 232-630-9
• Número MDL: MFCD00131847
• Código UNSPSC: 12352204

Propiedades

• Formulario: ammonium sulfate suspension
• actividad específica: ≥200 units/mg protein (biuret)
• mol peso: 111 kDa
• actividad extraña: Apyrase (ATPase) ≤5% of base activity
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
• Clave InChI: GZCWLCBFPRFLKL-UHFFFAOYSA-N

Descripción

Descripción general

Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.

Aplicación

Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.

Acciones bioquímicas o fisiológicas

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Definición de unidad

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Forma física

Suspension in 1.8 M (NH₄)₂SO₄, 10 mM MgCl₂, pH 5.5

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)