This product is lyophilized from a solution containing phosphate buffer and sucrose and may be reconstituted in water. For testing purposes, this product is dissolved at 5 mg/mL in a cold assay buffer. See below to review the composition of the 50 mM Tris assay buffer:
1.0 ml 1 M Tris stock solution
0.4 ml of 0.5 M magnesium chloride stock solution
0.01 ml Mercapto-ethanol
Add to 15 ml H2O
adjust pH 7.3 with HCl 1 M
QS to 20 ml
48275
β-Galactosidasa from Escherichia coli
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
Sinónimos:
β-D-galactósido galactohidrolasa, Lactasa
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
1 MG
MXP 5,197.00
25 MG
MXP 8,682.00
5 MG
MXP 10,686.00
MXP 5,197.00
Fecha estimada de envío31 de mayo de 2025
Seleccione un Tamaño
Cambiar Vistas
1 MG
MXP 5,197.00
25 MG
MXP 8,682.00
5 MG
MXP 10,686.00
About This Item
MXP 5,197.00
Fecha estimada de envío31 de mayo de 2025
Productos recomendados
Formulario
powder
calidad
lyophilized
actividad específica
~140 U/mg
mol peso
Mr ~135000
contiene
phosphate buffer as stabilizer
sucrose as stabilizer
técnicas
enzyme immunoassay: suitable
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Aplicación
Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
Acciones bioquímicas o fisiológicas
βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.
Envase
Bottomless glass bottle. Contents are inside inserted fused cone.
Propiedades físicas
Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)
Definición de unidad
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C
Otras notas
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)citation; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay techniquecitation; Enzyme immunoassay of thyrptropin releasing hormone (TRH)citation; Preparation of enzyme-antibody conjugatescitation
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)[1]; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique[2]; Enzyme immunoassay of thyrptropin releasing hormone (TRH)[3]; Preparation of enzyme-antibody conjugates[4]
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
Christophe Laumonnerie et al.
Development (Cambridge, England), 141(3), 594-603 (2014-01-23)
The establishment of anatomically stereotyped axonal projections is fundamental to neuronal function. While most neurons project their axons within the central nervous system (CNS), only axons of centrally born motoneurons and peripherally born sensory neurons link the CNS and peripheral
-
I have already bought this one. My question is how will I reconstitute it? What diluent or buffer will I use?
1 answer-
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
