10105031001
Roche
β-Galactosidase
from E. coli overproducer
Sinónimos:
β-galactosidase
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1500 UNITS
MXP 3,932.00
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1500 UNITS
MXP 3,932.00
About This Item
MXP 3,932.00
Check Cart for Availability
Productos recomendados
origen biológico
Escherichia coli
Nivel de calidad
Formulario
suspension
mol peso
540 kDa
envase
pkg of 1,500 U
fabricante / nombre comercial
Roche
concentración
5.00 mg/mL
técnicas
activity assay: suitable
pH óptimo
7
idoneidad
suitable for UV spectrophotometry and general use
aplicaciones
life science and biopharma
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
β-Galactosidase or β-D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. β-Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with β-d-galactopyranosides.[1]
At 37 °C with 2-nitrophenyl-β-D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.
Especificidad
Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.
Aplicación
β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA).[2] Use β-Galactosidase to produce a calibration curve in enzymatic assays.
Calidad
Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each
Forma física
Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline
Nota de preparación
The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.
In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.
In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
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