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P0029

Protease from Bacillus sp.

Synonym(s):

Protamex®

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About This Item

EC Number:
3.4.21.14
UNSPSC Code:
12352204
EC Number:
232-909-5
NACRES:
NA.54
MDL number:
Biological source:
Bacillus sp.
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biological source

Bacillus sp.

Quality Level

form

granular

enzyme activity

>1.5 AU-N/g

storage temp.

2-8°C

General description

Protease from Bacillus sp. is extensively useful in pharmaceutical, leather, food and waste processing industry. It is widely used in manufacturing protein hydrolysates. Analysis of Bacillus protease stability and activity showed that the enzyme was active at all pH values from 5.0-11.0 with 100% activity at 8.0. Optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.

Application

Protease from Bacillus sp. has been used:
  • to evaluate protease inhibitor activity
  • in high yields in batch fermentation using a response surface methodology[1]
  • in a study to investigate a biosensor to detect a protease biomarker from Bacillus licheniformis[2]

Analysis Note

minimum activity 1.5 AU-N/G solid

Legal Information

Protamex is a registered trademark of Novozymes Corp.

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This Item
P3111P5380P1236
biological source

Bacillus sp.

biological source

Bacillus sp.

biological source

Bacillus licheniformis

biological source

Bacillus sp. (Bacillus amyloliquefaciens)

form

granular

form

liquid

form

lyophilized powder

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

enzyme activity

>1.5 AU-N/g

enzyme activity

-

enzyme activity

-

enzyme activity

-


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Huiwang Wu et al.
The Analyst, 137(20), 4829-4833 (2012-08-30)
A simple, selective and sensitive electrochemical biosensor has been developed to detect protease biomarker from Bacillus licheniformis, a recognized model of the biochemical warfare agent Bacillus anthracis. In this assay, the biosensor is constructed using a d-amine acid containing substrate
Hypocholesterolemic effect of compounded freshwater clam protein hydrolysate and Gracilaria
Lin, Y-H, et al
Food Chemistry, 123(2), 395-399 (2010)
Trypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial Origin
<BIG>Ali PPM, et al.</BIG>
Applied Biochemistry and Biotechnology, 167-178 (2014)



Global Trade Item Number

SKUGTIN
P0029-50G04061834261902
P0029-250G04061834261896

Questions

1–4 of 4 Questions  
  1. Podrían decirme qué diámetro de partícula tiene, por favor

    1 answer
    1. The particle size of this material has not been investigated.

      Helpful?

  2. What is the density of this protease?

    1 answer
    1. This product is a granulated preparation; the density has not been determined.

      Helpful?

  3. Podrían indicarme las temperaturas y pH óptimos de trabajo de esta proteasa

    1 answer
    1. As mentioned in the 'DESCRIPTION' section, the optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.

      Helpful?

  4. What is the composition (or protein content) of this protease?

    1 answer
    1. This is a product of Novozymes. The composition is considered proprietary, however, it is a blend of aggressive endo-proteases.

      See the link below to a publication that may be helpful:
      https://www.sciencedirect.com/science/article/pii/S2772502222000683

      Helpful?

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