FCANOS1
Nitric Oxide Synthase Detection System, Fluorimetric
sufficient for 200 reactions
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352303
Pricing and availability is not currently available.
usage
sufficient for 200 reactions
Quality Segment
availability
not available in Japan
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... NOS1(4842), NOS2(4843), NOS2B(201288), NOS2C(645740), NOS3(4846)
Biochem/physiol Actions
Simple and specific assay for the measurement of free nitric oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Nitric oxide, formed from the amino acid L-arginine via the action of NOS, is a unique cell signaling molecule that functions as both an intracellular and an extracellular messenger. NO has been implicated in vasodilation, neurotransmission, cytotoxicity, and inflammation.
The standard assay for NOS activity is for solubilized cell-free enzyme preparations such as cell lysates, crude tissue extracts or purified enzymes. It measures the production of radiolabeled citrulline from radiolabeled arginine substrate (Sigma Product No. NOS-1). In contrast, the Fluorometric Cell-Associated NOS Detection System measures intracellular production of NO by a non-radiometric method. Radioisotopes are replaced by a cell-permeable diacetate derivative of 4,5-diaminofluorescein (DAF-2 DA). DAF-2 DA penetrates cells rapidly where it is hydrolyzed by intracellular esterase to DAF-2 that, in turn, reacts with NO produced by NOS to form a fluorescent triazolo-fluorescein. The fluorescent product can be quantitated using an excitation filter at 492 nm and an emission filter at 515 nm.
The standard assay for NOS activity is for solubilized cell-free enzyme preparations such as cell lysates, crude tissue extracts or purified enzymes. It measures the production of radiolabeled citrulline from radiolabeled arginine substrate (Sigma Product No. NOS-1). In contrast, the Fluorometric Cell-Associated NOS Detection System measures intracellular production of NO by a non-radiometric method. Radioisotopes are replaced by a cell-permeable diacetate derivative of 4,5-diaminofluorescein (DAF-2 DA). DAF-2 DA penetrates cells rapidly where it is hydrolyzed by intracellular esterase to DAF-2 that, in turn, reacts with NO produced by NOS to form a fluorescent triazolo-fluorescein. The fluorescent product can be quantitated using an excitation filter at 492 nm and an emission filter at 515 nm.
Legal Information
DAF-2 DA is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874, 590
US Patent No. 5,874, 590
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This Item | |||
|---|---|---|---|
| storage temp. 2-8°C | storage temp. - | storage temp. −70°C | storage temp. −20°C |
| availability not available in Japan | availability - | availability - | availability - |
| Quality Level 200 | Quality Level EQ2 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 200 reactions | usage - | usage - | usage - |
| shipped in wet ice | shipped in - | shipped in dry ice | shipped in dry ice |
| Gene Information human ... NOS1(4842), NOS2(4843), NOS2B(201288), NOS2C(645740), NOS3(4846) | Gene Information - | Gene Information mouse ... Nos2(18126) | Gene Information mouse ... Nos2(18126) |
Kit Components Only
Product No.
Description
- Diphenyleneiodonium Chloride (DPI) 1 mM 200 μL
- Arginine Substrate Solution for NOS Detection, Fluorimetric 2 mL
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| FCANOS1-1KT | 04061833622315 |