F0635
Rat Fibronectin
from rat plasma, powder, suitable for cell culture
Synonym(s):
Fibronectin
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About This Item
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product name
Fibronectin rat plasma, powder, BioReagent, suitable for cell culture
biological source
rat plasma
Quality Level
sterility
sterile
product line
BioReagent
form
powder
packaging
pkg of 0.5 mg
pkg of 1 mg
pkg of 2 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
solubility
H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)
UniProt accession no.
Binding Specificity
Peptide Source: Collagen
shipped in
dry ice
storage temp.
−20°C
Gene Information
rat ... Fn1(25661)
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General description
Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.
Application
Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation and primary neuronal ventral mesencephalon cultures.
Biochem/physiol Actions
Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.
Components
Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.
Caution
The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Preparation Note
This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
Analysis Note
A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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