Key Documents

View All Documentation

3,3′-Diaminobenzidine tetrahydrochloride

Name: 3,3&#8242;-Diaminobenzidine tetrahydrochloride
Brand: SIGMA

peroxidase substrate, chromogenic, tablet

Synonym(s):

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

Select a Size

Change View

Pack Size	SKU	Availability	Price

About This Item

Linear Formula:

(NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl

Molecular Weight:

360.11

NACRES:

NA.83

PubChem Substance ID:

24893981

UNSPSC Code:

12352204

EC Number:

231-018-9

MDL number:

MFCD00012969

Beilstein/REAXYS Number:

1212988

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

Quality Segment

200

form

tablet

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

storage temp.

−20°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

Description

Application

Reagent for spectrophotometric determination of selenium.

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Biochem/physiol Actions

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Preparation Note

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	T3405	D8001	D12384
Sigma-Aldrich D5905 3,3′-Diaminobenzidine tetrahydrochloride	Sigma-Aldrich T3405 3,3′,5,5′-Tetramethylbenzidine dihydrochloride	Sigma-Aldrich D8001 3,3′-Diaminobenzidine	Sigma-Aldrich D12384 3,3′-Diaminobenzidine
solubility water: 1 tablet/15 mL (may contain small insoluble particles)	solubility -	solubility -	solubility -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form tablet	form tablet	form powder	form powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. room temp	storage temp. -