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Human Collagen Type IX

Name: Human Collagen Type IX
Brand: SIGMA

from human placenta, powder, suitable for detection assays

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About This Item

CAS Number:

9007-34-5

UNSPSC Code:

12352202

NACRES:

NA.32

EC Number:

232-697-4

MDL number:

MFCD00130825

Form:

powder

Biological source:

human placenta

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Properties

Product Name

Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder

biological source

human placenta

Quality Level

200

form

powder

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

aqueous acid: soluble

UniProt accession no.

P20908

application(s)

detection

storage temp.

2-8°C

Gene Information

human ... COL5A1(1289)

Description

Preparation Note

This product was prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Disclaimer

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

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1 of 1

This Item	C8374	C5608	C1188
Sigma-Aldrich C3657 Collagen from human placenta	Sigma-Aldrich C8374 Collagen from human placenta	Sigma-Aldrich C5608 Collagen from rabbit skin	Sigma-Aldrich C1188 Collagen from bovine tracheal cartilage
biological source human placenta	biological source human placenta	biological source rabbit skin	biological source bovine cartilage (tracheal)
form powder	form powder	form powder	form powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities HIV, hepatitis B and hepatitis C, none detected	impurities HIV, hepatitis B and hepatitis C, none detected	impurities -	impurities -
Gene Information human ... COL5A1(1289)	Gene Information human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)	Gene Information rabbit ... COL1A2(100008997)	Gene Information cow ... COL2A1(407142)
solubility aqueous acid: soluble	solubility 0.5 M acetic acid: 1 mg/mL, clear to very slightly hazy	solubility aqueous acid: ≤10 mg/mL, aqueous sodium acetate buffer: soluble (0.01-0.5M)	solubility aqueous acid: soluble

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
C3657-5MG	04061833260920
C3657-1MG	04061833486177

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