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A3641

Apoferritin from equine spleen

0.2 μm filtered

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About This Item

CAS Number:
9013-31-4
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00081365
Form:
liquid
Mol wt:
major subunit ML 19,889, minor subunit MH 22,200, native ~481.2 kDa (24 subunits, approx. 20 kDa each)
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Quality Segment

200

sterility

0.2 μm filtered

form

liquid

mol wt

major subunit ML 19,889, minor subunit MH 22,200, native ~481.2 kDa (24 subunits, approx. 20 kDa each)

color

clear to slightly hazy, solution

storage temp.

2-8°C

Application

Apoferritin from equine spleen has been used:
  • to determine its partition coefficients in phase systems
  • in NaCl for iron loading of cultured cells
  • in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology

Biochem/physiol Actions

This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis.[1] It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant.[2] When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis. [3]

Physical form

Solution in 0.135 M sodium chloride.

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This Item
F6754A9460V8379
Apoferritin from equine spleen 0.2 μm filtered

Sigma-Aldrich

A3641

Apoferritin from equine spleen

Ferritin from human liver Type IV, 10 μg/mL

Sigma-Aldrich

F6754

Ferritin from human liver

Annexin V from human placenta ≥90% (SDS-PAGE), buffered aqueous solution

Sigma-Aldrich

A9460

Annexin V from human placenta

Vitronectin from human plasma lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

V8379

Vitronectin from human plasma

mol wt

major subunit ML 19,889, native ~481.2 kDa (24 subunits, approx. 20 kDa each), minor subunit MH 22,200

mol wt

H subunit ~21 kDa, L subunit ~19 kDa, ~440 kDa (a shell of 24 protein subunits (apoferritin) and a core of Fe3+ ions)

mol wt

33 kDa (four subunits)

mol wt

A subunit 75 kDa, B subunit 65 kDa

form

liquid

form

liquid

form

buffered aqueous solution

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

300

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

-

color

clear to slightly hazy, solution

color

-

color

-

color

-

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000472484
0000472484
0000408416
0000368426
0000344224

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Global Trade Item Number

SKUGTIN
A3641-1G04061826740873
A3641-500MG04061833258118
A3641-100MG04061833367704

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