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Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

Name: Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

Synonym(s):

Anti-Nuclear Factor ΚB

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About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

human

Application:

—

Citations:

Pricing and availability is not currently available.

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Properties

biological source

rabbit

Quality Level

100

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA
Gel Shift
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation )

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.

Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.

This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Immunogen

Human

a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH

Application

ELISA (see comments)

Gel Shift (see comments)

Immunoblotting (1:500, chemiluminescence)

Immunoprecipitation (see comments)

Physical form

In 5 mM sodium phosphate buffer, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Daudi cells

Other Notes

Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.

This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Show Differences

1 of 1

This Item	N8523	SAB4502617	SAB4502609
Sigma-Aldrich PC138 Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb	Sigma-Aldrich N8523 Monoclonal Anti-NF-κB antibody produced in mouse	Sigma-Aldrich SAB4502617 Anti-NF-κB p65 antibody produced in rabbit	Sigma-Aldrich SAB4502609 Anti-NF-κB p65 antibody produced in rabbit
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
clone polyclonal	clone NF-12, monoclonal	clone polyclonal	clone polyclonal
form liquid	form -	form buffered aqueous solution	form buffered aqueous solution
species reactivity human	species reactivity human, mouse	species reactivity human, mouse	species reactivity human, mouse

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
PC138-100UL	04055977209464

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