AP192B

Donkey Anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed

0.8 mg/mL (after reconstitution), Chemicon^®

• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: biotin conjugate
• Clone: polyclonal
• Application: ELISA, WB
• Citations: 18

Properties

• biological source: donkey
• Quality Level: 100
• conjugate: biotin conjugate
• antibody form: F(ab′)₂ fragment of affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• concentration: 0.8 mg/mL (after reconstitution)
• technique(s): ELISA: suitable, western blot: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

Donkey anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed detects level of Donkey Mouse IgG & has been published & validated for use in ELISA & WB.

EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Biochem/physiol Actions

Specific for mouse IgG, heavy and light chain.

Physical form

Immunoaffinity Purified and Immunodepletion

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA and 0.01% thimerosal.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

Preparation Note

Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3