ABD69
Anti-Nestin, Human Antibody
serum, from rabbit
Synonym(s):
Nestin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, WB
Citations:
74
Pricing and availability is not currently available.
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
should not react with
rat, mouse
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NES(10763)
General description
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
The anti-Nestin antibody has an observed MW at 220kDa Antibody may see 260kDa band in some cells and/or treatments
Immunogen
GST-tagged recombinant protein corresponding to human Nestin.
Application
Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC.
Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.
Biochem/physiol Actions
This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein.
Analysis Note
Control
U251 glioblastoma cells.
U251 glioblastoma cells.
Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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This Item | |||
|---|---|---|---|
| antibody form serum | antibody form purified antibody | antibody form purified antibody | antibody form purified immunoglobulin |
| biological source rabbit | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate PE | conjugate ALEXA FLUOR™ 488 | conjugate biotin conjugate |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| species reactivity human | species reactivity human | species reactivity human | species reactivity mouse, rat |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABD69 | 04053252694288 |