跳转至内容

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

摘要

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

材料

货号

品牌

产品描述

Sigma-Aldrich

二甲基亚砜, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Sigma-Aldrich

丙酮, ACS reagent, ≥99.5%

Sigma-Aldrich

二甲基亚砜, ACS reagent, ≥99.9%

Sigma-Aldrich

氧化氘, 99.9 atom % D

Sigma-Aldrich

二甲基亚砜, for molecular biology

Sigma-Aldrich

二甲基亚砜, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Sigma-Aldrich

二甲基亚砜, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

1,2-二氯乙烷, ACS reagent, ≥99.0%

Sigma-Aldrich

丙酮, Laboratory Reagent, ≥99.5%

Sigma-Aldrich

二甲基亚砜, ≥99.5% (GC), suitable for plant cell culture

Sigma-Aldrich

二甲基亚砜, puriss. p.a., ACS reagent, ≥99.9% (GC)

Sigma-Aldrich

N-羟基丁二酰亚胺, 98%

Sigma-Aldrich

L-谷氨酰胺, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

Sigma-Aldrich

二甲基亚砜, anhydrous, ≥99.9%

Sigma-Aldrich

氧化氘, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

Sigma-Aldrich

L-谷氨酰胺

Sigma-Aldrich

丙酮, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

Sigma-Aldrich

二甲基亚砜, BioUltra, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

丙酮, ACS reagent, ≥99.5%

Sigma-Aldrich

N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐, ≥99.0% (AT)

SAFC

L-谷氨酰胺

Sigma-Aldrich

N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐, commercial grade, powder

Sigma-Aldrich

1,2-二氯乙烷, anhydrous, 99.8%

Sigma-Aldrich

四丁基氢氧化铵溶液, 40 wt. % in H₂O

Sigma-Aldrich

丙酮, histological grade, ≥99.5%

Sigma-Aldrich

N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐, crystalline

Sigma-Aldrich

四丁基氢氧化铵溶液, 1.0 M in methanol

Sigma-Aldrich

二乙胺, ≥99.5%

Sigma-Aldrich

丙酮, JIS special grade, ≥99.5%

Sigma-Aldrich

二甲基亚砜, puriss. p.a., dried, ≤0.02% water