ZRB1817

Anti-ASS1 Antibody, clone 2E22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC代码: 12352203
• NACRES: NA.43

属性

• 生物来源: rabbit
• 质量水平: 200
• 重组: expressed in HEK 293 cells
• 偶联物: unconjugated
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: 2E22, recombinant monoclonal
• 描述: 2E22 Clone
• 产品线: ZooMAb^® learn more
• 表单: lyophilized
• 分子量: calculated mol wt 46.53 kDa; observed mol wt ~46 kDa
• 纯化方式: using Protein A
• 种属反应性: mouse, human
• 种属反应性（根据同源性预测）: porcine, chimpanzee, bovine
• 包装: antibody small pack of 25 μL
• 环保替代产品特性: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 增强验证: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技术: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同位素/亚型: IgG
• 表位序列: Internal
• Protein ID登记号: NP_000041
• UniProt登记号: P00966
• 环保替代产品分类: , Aligned
• 运输: ambient
• 储存温度: 2-8°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... ASS1(445)

说明

一般描述

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 2E22 is a C13 Arginosuccinate synthase (ASS1). It targets an epitope within 24 amino acids from the internal region.

免疫原

KLH-conjugated linear peptide corresponding to 24 amino acids from the internal region of human Arginosuccinate synthase (ASS1).

应用

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Arginosuccinate Synthase (ASS1) in HepG2 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ASS1 in Raji cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound ASS1 with a KD of 1.3 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ASS1 in NIH3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ASS1 in human kidney tissue sections.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected ASS1 in one million Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Arginosuccinate synthase (UniProt: P00966; also known as EC:6.3.4.5, Citrulline--aspartate ligase) is encoded by the ASS1 gene (Gene ID: 445) in human. Arginosuccinate synthase (ASS) is a cytosolic enzyme with highest expression in the liver, renal proximal tubules, and testis. In liver, it is distributed homogeneously and is not confined to periportal cells. In liver, argininosuccinate synthase is reported to associate with the outside of the mitochondria to complete the urea cycle, which involves both the mitochondrial and cytosolic compartments. ASS is a rate-limiting enzyme in the urea cycle. It catalyzes the formation of arginosuccinate from aspartate, citrulline, and ATP. ASS is shown to participate in five major pathways including urea synthesis, nitric oxide synthesis, polyamine synthesis, creatine synthesis, and de novo synthesis of arginine to maintain serum levels. Mutations in ASS1 gene has been linked to citrullinemia type I deficiency that is characterized primarily by elevated serum and urine citrulline levels and ammonia intoxication that is usually manifested in the first few days of life. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Haines, RJ., et al. (2011). Int. J. Biochem. Mol. Biol. 2(1); 8-23; Goodwin, BL., et al. (2004). J. Biol. Chem. 279(18); 18353-18360).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

储存及稳定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 13 - Non Combustible Solids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable