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Key Documents

ZRB1377

Sigma-Aldrich

Anti-EZHIP/CXorf67 Antibody, clone 1J8 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

别名:

Anti-CATACOMB, Anti-CXorf67, Anti-KIP75

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

重組細胞

expressed in HEK 293 cells

共軛

unconjugated

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

1J8, recombinant monoclonal

描述

recombinant, expressed in HEK 293 cells

產品線

ZooMAb® learn more

形狀

lyophilized

分子量

calculated mol wt 51.89 kDa
observed mol wt ~52 kDa

物種活性

human

包裝

antibody small pack of 25 μL

環保替代產品特色

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

加強驗證

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

技術

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

同型

IgG

表位序列

N-terminal half

Protein ID登錄號

UniProt登錄號

環保替代類別

運輸包裝

ambient

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1J8 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects EZH Inhibitory protein (EZHIP). It targets an epitope within 18 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human EZH Inhibitory Protein (EZHIP).

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells transfected with EZHIP.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected EZHIP/CXorf67 in lysate from HEK293 cells stably transfected with N-terminal FLAG-tagged human EZHIP.

Tested applications


Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected EZHIP/CXorf67 in human testis tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZHIP/CXorf67 in U2OS cell line.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-EZHIP/CXorf67, clone 1J8 ZooMAb®, Cat. No. ZRB1377, is a recombinant Rabbit monoclonal antibody that specifically detects EZHIP/CXorf67 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

標靶描述

EZH inhibitory protein (UniProt: Q86X51; also known as EZHIP) is encoded by the EZHIP (also known as CXorf67) gene (Gene ID: 340602) in human. EZHIP is expressed predominantly in the gonads. In testis, it is detected in male germ cells inside the seminiferous tubules, especially in spermatogonia and round spermatids. In the ovary, it is expressed in primordial follicles and oocytes but not the external follicle cells. It is shown to inhibit PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation on lysine 27 (H3K27me3). It inhibits H3K27me3 deposition during spermatogenesis and oogenesis. Its inhibitory effect on methyltransferase activity is attributed to its ability to limit the stimulatory effect of cofactors such as AEBP2 and JARID2. Amino acid sequence 403-423 is reported to be essential and sufficient for inhibition of PRC2/EED-EZH1 and PRC2/EED-EZH2 complex activity and the sequence 401-409 is sufficient for its interaction with EZH2. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ragazzini, R., et al. (2019). Nat. Commun. 10(1); 3858).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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