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Merck

XNAR

Sigma-Aldrich

Extract-N-Amp植物PCR试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

别名:

Plant direct PCR kit

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About This Item

UNSPSC代码:
41106303
NACRES:
NA.55

用途

sufficient for 1000 amplifications
sufficient for 1000 extractions

特点

hotstart

技术

PCR: suitable

颜色

colorless

运输

wet ice

储存温度

−20°C

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一般描述

Extract-N-Amp 植物PCR试剂盒含有从植物叶片中快速提取基因组DNA并通过PCR反应扩增感兴趣的目标基因必需的所有试剂。使用新型Extraction Solution,无需通过液氮对植物组织进行传统的冰冻,机械式破碎、有机萃取或沉淀DNA操作。该试剂盒同时包含一种专为从提取物直接扩增而配制的PCR ReadyMix

基因组DNA提取自0.5至0.7 cm的植物叶盘,该叶盘通过标准打孔器获得并在95 oC下Extraction Solution中孵育10分钟。向提取物中加入等体积的Dilution Solution,以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR ReadyMix(包含在试剂盒内)的PCR反应物中。
Extract-N-Amp植物PCR试剂盒用于直接PCR(聚合酶链反应)。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案,无需常规的植物组织冷冻处理。

应用

Extract-N-Amp植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。
Extract-N-Amp组织PCR试剂盒用于从新鲜蘑菇中提取DNA。

特点和优势

  • 可在15分钟内一步提取出用于PCR反应的植物基因组DNA
  • 专为直接从提取物进行扩增而配置的PCR ReadyMix
  • 适用于基因组DNA的高度特异性PCR扩增的热启动抗体
  • 提取物可在4 °C 下至少稳定保存6个月
  • PCR用植物基因组DNA可以在15分钟内提取。
  • 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
  • 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
  • 热启动抗体确保基因组DNA的高度特异性PCR扩增
  • 适用于高通量植物基因分析
  • 无需长时间的酶消化,节省时间和精力
  • 提取的DNA在4 °C 下至少6个月保持稳定。

组分

Extract-N-Amp植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物(2X)(缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体)。

其他说明

Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。
更多信息,请见www.sigma-aldrich.com/extract-n-amp

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

象形图

Exclamation markEnvironment

警示用语:

Warning

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Alison Heffer et al.
EvoDevo, 2(1), 7-7 (2011-03-03)
Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family
Rapid and reliable high-throughput methods of DNA extraction for use in barcoding and molecular systematics of mushrooms
Dentinger BTM, et al.
Molecular Ecology Resources, 10(4), 628-633 (2010)
M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Jian-Feng Li et al.
Plant methods, 6(1), 1-1 (2010-02-26)
Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed
Matthew E Smith et al.
PloS one, 8(1), e55160-e55160 (2013-02-06)
Ectomycorrhizal (ECM) plants and fungi can be diverse and abundant in certain tropical ecosystems. For example, the primarily paleotropical ECM plant family Dipterocarpaceae is one of the most speciose and ecologically important tree families in Southeast Asia. Pakaraimaea dipterocarpacea is

商品

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

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实验方案

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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