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Merck
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主要文件

G2N70

Sigma-Aldrich

GenElute™ 植物基因组 DNA 微量制备试剂盒

greener alternative

sufficient for 70 purifications

别名:

植物基因组DNA少量制备, Gen Elute

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.55

用途

sufficient for 70 purifications

环保替代产品特性

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

DNA purification: suitable

环保替代产品分类

, Aligned

储存温度

15-25°C

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相关类别

一般描述

GenElute植物基因组DNA制备试剂盒可以简单、方便的从各种植物中分离高纯度的基因组DNA。该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、RNase处理，以及诸如苯酚和氯仿等有害化学成分。

首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。

纯化所得的DNA可直接用于PCR、限制性酶切、克隆、测序和Southern印迹等下游应用。
GenElute植物基因组DNA小量制备试剂盒可以简单、方便的从各种植物中分离高纯度的DNA。GenElute试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、RNase处理，以及诸如苯酚和氯仿等有害化学成分。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，本品是一种本安型化学制剂。

应用

纯化所得的高纯度DNA可直接供以下敏感的下游应用使用：
  • PCR
  • 限制性内切酶酶切
  • 克隆
  • Southern印迹
纯化的基因组DNA可立即用于敏感的下游应用，如：
  • PCR
  • 限制性内切酶酶解
  • 克隆
  • Southern印迹

GenElute™植物基因组DNA Miniprep试剂盒用于：
  • 纯化从楝树组织中分离的基组DNA
  • 从胚胎中提取基因组DNA
  • 从植物样本中分离DNA

特点和优势

  • 起始材料：不超过100 mg的植物组织
  • 预期得率：至多20 μg
  • 洗脱体积：100 - 200 μl
  • 所需时间：<40分钟
  • 是否需要RNase处理：否

原理

在1小时内，可从至多100 mg新鲜组织或10 mg冻干材料中提取出微克级的DNA。首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。纯化所得DNA长度超过20 kb。

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C

补充剂危害

EUH032

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000401715
0000401715
SLCL4188
SLCH0037
SLCD2039

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在文件库中查找您最近购买产品的文档。

访问文档库

Comparative Analysis of the Genomic DNA Isolation Methods on Inula sp.(Asteraceae)
SEViNDiK E, et al.
Notulae Scientia Biologicae , 8(4), 451-455 (2016)
The optimization of voltage parameter for tissue electroporation in somatic embryos of Astragalus chrysochlorus (Leguminosae)
Turgut-Kara N and Ari S
African Journal of Biotechnology, 9(29), 4584-4588 (2010)
Václav Bačovský et al.
International journal of molecular sciences, 23(5) (2022-03-11)
Young sex chromosomes possess unique and ongoing dynamics that allow us to understand processes that have an impact on their evolution and divergence. The genus Silene includes species with evolutionarily young sex chromosomes, and two species of section Melandrium, namely
Yul-Kyun Ahn et al.
Scientific reports, 8(1), 5188-5188 (2018-03-28)
The present study deals with genome wide identification of single-nucleotide polymorphism (SNP) markers related to powdery mildew (PM) resistance in two pepper varieties. Capsicum baccatum (PRH1- a PM resistant line) and Capsicum annuum (Saengryeg- a PM susceptible line), were resequenced
M D Bennett et al.
Annals of botany, 107(3), 467-590 (2011-01-25)
The amount of DNA in an unreplicated gametic chromosome complement is known as the C-value and is a key biodiversity character of fundamental significance with many practical and predictive uses. Since 1976, Bennett and colleagues have assembled eight compilations of
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Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ Plant PCR Kits

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

实验方案

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

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Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

DNA和RNA纯化

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