跳转至内容
Merck
所有图片(1)

Key Documents

View All Documentation

XNAB2R

Sigma-Aldrich

Extract-N-Amp™血液PCR试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

别名:

血液直接 PCR 试剂盒

登录查看公司和协议定价
所有图片(1)

About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 1000 amplifications
 sufficient for 1000 extractions
 sufficient for 1000 reactions

特點

dNTPs included
hotstart

技術

PCR: suitable

顏色

colorless

運輸包裝

wet ice

儲存溫度

−20°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

Extract-N-Amp™血液PCR试剂盒提供了从全血快速提取DNA以及通过直接PCR扩增靶标所需的所有试剂。这种新型提取系统无需进行任何类型的纯化、有机提取、离心、加热、过滤或酒精沉淀。该试剂盒还包括专门配制的PCR ReadyMix™，可直接从提取物中扩增。这种配方使用基于抗体的热启动，用于特异性扩增。

應用

Extract-N-Amp™血液PCR试剂盒已用于从基因组和干血斑中提取DNA。还用于聚合酶链式反应（PCR）。

特點和優勢

  • 高效8分钟预制品允许更大的速度和吞吐量
  • 不需要任何类型的净化、有机提取、离心或酒精沉淀
  • 简单的3步程序，无需特殊设备
  • 包括Hot start抗体，用于基因组DNA的高特异性PCR扩增
  • 兼容多种格式（单管或96孔板）
  • 可与全血或血卡一起使用
  • 在4℃稳定提取至少6个月

原則

基因组DNA从10 μl全血中提取，只需加入提取液并在室温下孵育5分钟。PCR前，将中和液添加至提取液以中和抑制物质。然后将一部分DNA提取物添加至专门配制的PCR Mix的PCR ReadyMix中。

其他說明

有关其他信息，请参阅www.sigma-aldrich.com/extract-n-amp。

法律資訊

购买本产品包括豁免根据产品说明书中所规定的专利提起的诉讼，仅可将所购买的量用于购买者自己的内部研究。′其他专利权（例如5′核酸酶工艺的专利权）不得明示、暗示或禁止反言。 有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
根据美国专利号5,338,671和5,587,287以及其他国家的相应专利批准用于体外研究的抗体。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • L3289Lysis Solution for Blood化学品安全说明书
  • N9784Neutralization Solution for Blood化学品安全说明书
  • P8115Extract-N-Amp™ PCR ReadyMix™ for Blood化学品安全说明书

儲存類別代碼

10 - Combustible liquids

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Mads Vilhelm Hollegaard et al.
Electrophoresis, 30(14), 2532-2535 (2009-07-30)
Stored surplus of dried blood spot (DBS) samples from neonatal screening programs constitute a vast potential for large genetic epidemiological studies. However, age of the samples and the small amounts of DNA available may limit their usage. In this study
Nicholas Wong et al.
BioTechniques, 45(4), 423-424 (2008-10-16)
Sodium bisulfite treatment followed by PCR and DNA sequencing is widely considered the gold standard for the analysis of DNA methylation patterns. However, this technique generally requires substantial quantities of genomic DNA as starting material and is often associated with
Mads V Hollegaard et al.
BMC genomics, 10, 297-297 (2009-07-07)
Identification of disease susceptible genes requires access to DNA from numerous well-characterised subjects. Archived residual dried blood spot samples from national newborn screening programs may provide DNA from entire populations and medical registries the corresponding clinical information. The amount of
Jun Shi et al.
Cancer research, 67(13), 6417-6424 (2007-07-10)
Idiopathic myelofibrosis (IM) is likely the consequence of both the acquisition of genetic mutations and epigenetic changes that silence critical genes that control cell proliferation, differentiation, and apoptosis. We have explored the effects of the sequential treatment with the DNA
Sandrine Romand et al.
Biotechnology and bioengineering, 113(5), 1094-1101 (2015-11-03)
Chinese Hamster Ovary (CHO) cells are widely used for the large-scale production of recombinant biopharmaceuticals. However, attempts to express IGF-1 (a mutated human Insulin-like growth factor 1 Ea peptide (hIGF-1Ea mut)) in CHO cells resulted in poor cell growth and
查看所有相关文献

商品

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门