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W1754

Sigma-Aldrich

水

PCR Reagent, suitable for PCR

别名:

H2O

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About This Item

线性分子式:
H2O
CAS号:
7732-18-5
分子量:
18.02
Beilstein:
2050024
EC 号:
231-791-2
MDL编号:
MFCD00011332
UNSPSC代码:
12191602
PubChem化学物质编号:
24900779
NACRES:
NA.25

产品名称

水, PCR Reagent

蒸汽密度

<1 (vs air)

蒸汽压

3 mmHg

无菌性

sterile-filtered

表单

liquid

包装

vial of 1.5 mL

技术

PCR: suitable

折射率

n20/D 1.34 (lit.)

pH值（酸碱度）

5-7

沸点

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

异质活性

DNase, none detected
RNase, none detected

SMILES字符串

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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相关类别

一般描述

对 PCR 级水进行无菌过滤。它不含核酸外切酶（dna 酶、rna 酶）和核酸内切酶（切口酶），也不含核酸污染。

应用

水已用于：

  • 作为反应混合物的组分和微流控RT-qPCR的稀释剂
  • 作为反应混合物的组分，以扩增真菌（杂色云芝）DNA的产物
  • 作为稀释剂和反应混合物的组分，以扩增cDNA
  • 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积

适用性

适用于聚合酶链式反应 (PCR)

其他说明

便于将水产品规格与 水规格表进行比较。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCP8635
SLCQ7402
SLCQ8885
SLCN7961
SLCP3569

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Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
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Journal of Applied Microbiology, 111(2), 484-490 (2011)
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