推荐产品
一般說明
REDExtract-N-Amp™血液PCR试剂盒提供了从全血快速提取DNA以及通过直接PCR（聚合酶链反应）扩增靶标所需的所有试剂。这种新型提取系统无需进行任何类型的纯化、有机提取、离心、加热、过滤或酒精沉淀。该试剂盒还包括专门配制的PCR ReadyMix™，可直接从提取物中扩增。这种配方使用基于抗体的热启动，用于特异性扩增。REDExtract-N-Amp血液PCR试剂盒中含有一种惰性染料，可作为示踪染料以实现方便地将PCR反应加载到琼脂糖凝胶上进行分析。
應用
REDExtract-N-Amp™血液PCR试剂盒已用于从血液样本、外周血白细胞、小鼠宫颈内拭子洗脱液和寄生虫中提取基因组DNA。它还用于通过寡核苷酸引物进行聚合酶链反应（PCR）。
特點和優勢
- 高效8分钟预制品允许更大的速度和吞吐量
- 不需要有机提取、离心或酒精沉淀
- Hot start抗体，用于基因组DNA的高特异性PCR扩增
- 可与全血或血卡一起使用
- 在4℃稳定提取至少6个月
- 兼容任何格式（单管、96孔等）
- 简单的3步程序，无需特殊设备
- REDExtract-N-Amp™不需要凝胶分析所需的装载缓冲液或跟踪染料
- PCR产品适用于直接克隆和测序
原則
只需加入Extraction Solution并在室温下孵育5分钟，即可从10 μl全血中提取出基因组DNA。向提取物中加入Neutralization Solution，以便在PCR反应前中和抑制物。随后选取一部分DNA提取物，加入专门配制的PCR ReadyMix。
其他說明
法律資訊
凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
相關產品
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
儲存類別代碼
8A - Combustible corrosive hazardous materials
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Lung-Cheng Lin et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 17(6), 1460-1469 (2008-06-19)
The urinary benzene metabolites, trans, trans-muconic acid (ttMA) and S-phenylmercapturic acid (SPMA), are widely used as benzene exposure biomarkers. The influence of the glutathione S-transferase (GST) genetic polymorphism on the excretion levels of urinary ttMA and/or SPMA has been investigated.
Olivier A E Sparagano et al.
Annals of the New York Academy of Sciences, 1081, 505-508 (2006-12-01)
Our study is based on the collection of blood and ticks from sheep in Iran and Italy. Polymerase chain reaction (PCR) testing was performed to target the 18S rRNA gene and RLB was performed using previously published probes. In Italy
Dawn M Reeves et al.
Antimicrobial agents and chemotherapy, 51(7), 2646-2648 (2007-05-16)
Mice treated with antibiotics early or late after active infection had resolved were examined for chlamydial DNA in endocervical swabs. The early eradication of infection limited oviduct pathology, despite the continued detection of chlamydial DNA by nested PCR. Late antibiotic
Anna K Nowińska et al.
Molecular vision, 17, 2333-2342 (2011-09-17)
To analyze genotype-phenotype correlation in patients originating from Polish population with the transforming growth factor beta induced (TGFBI) corneal dystrophies. Sixty affected and 31 unaffected individuals from 15 unrelated Polish families were included in the study. The clinical diagnosis was
Jun Liu et al.
Eukaryotic cell, 12(12), 1588-1599 (2013-07-23)
Microtubules are versatile biopolymers that support numerous vital cellular functions in eukaryotes. The specific properties of microtubules are dependent on distinct microtubule-associated proteins, as the tubulin subunits and microtubule structure are exceptionally conserved. Highly specialized microtubule-containing assemblies are often found
商品
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门