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X100RS

Sigma-Aldrich

Triton™ X-100（还原型）

别名:

聚氧代乙烯(10)异辛基环己基醚

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About This Item

线性分子式:
4-(C8H17)C6H10(OCH2CH2)nOH, n~10
CAS号:
92046-34-9
MDL编号:
MFCD00167048
UNSPSC代码:
12161900
NACRES:
NA.25
价格与库存信息目前不能提供

生物来源

synthetic (oragnic)

质量水平

100

描述

non-ionic

表单

viscous liquid

分子量

micellar average mol wt 80,000
average mol wt 625

聚集数

100-155

折射率

n20/D 1.473 (lit.)

CMC

0.2-0.9

mp

48 -50  °C (118 - 122 °F)

转变温度

cloud point 65 °C

溶解性

water: soluble, clear to slightly hazy, colorless

密度

1.029 g/mL at 25 °C (lit.)

吸附

≤0.25 at 277 nm in H2O at 0.5%

HLB

13.5

SMILES字符串

CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO

InChI

1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3

InChI key

QQJNBKDKLMCALZ-UHFFFAOYSA-N

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相关类别

一般描述

Triton™ X-100 还原剂是一种多功能的非离子表面活性剂，它具有亲水性聚环氧乙烷链，是一种常见的实验室去污剂。其应用涉及多种用途，包括裂解细胞以提取蛋白质或细胞器，透化活细胞膜，并可作为裂解缓冲液的重要成分。Triton™ X-100 在生物化学和细胞生物学研究中得到广泛应用，促进脂筏的分离，提高物质的可溶性和可分散性。该产品的还原聚氧乙烯含量为10左右，具有良好的润湿特性和乳化增强能力。

在细胞生物学中， Triton™ X-100 在膜结合蛋白的增溶中起关键作用，在溶液中保留了蛋白质的原始构象。此外， Triton™ X-100 还原剂是由TX-100的苯环部分完全氢化为环己烷衍生物而产生，它的潜在益处包括增进酶消化和对噬菌调理素的光致异构化的作用。Triton™ X-100在各种研究中的多功能性突出了它作为生物化学和细胞生物学研究中的基本工具的重要性。

应用

还原型 Triton™ X-100 已用于：
  • 核磁共振（NMR）样品管，用于裂解/破裂 1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）磷脂囊泡
  • 细胞内透化
  • 作为 Tris/HCl 中的一种组分，用于测定用于酶活性测定的酯的水解

特点和优势

  • 非离子表面活性剂
  • 增进物质的可溶性和可分散性
  • 良好的润湿特性
  • 增进乳化
  • 用于研究应用的高纯度产品

制备说明

氢化以降低UV吸光度。

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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说明
价格

象形图

Exclamation markEnvironment
GHS07,GHS09

警示用语：

Warning

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

235.4 °F - closed cup

闪点(°C)

113 °C - closed cup

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SHBN1375
SHBN4323
SHBQ0341
SHBN0704
SHBN0724

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H A Van Lith et al.
European journal of biochemistry, 206(2), 527-535 (1992-06-01)
1. Substrate hydrolysis by two purified rabbit liver esterase-1 allozymes (ES-1A and ES-1B) was compared under conditions differing in substrate, pH and temperature. ES-1A and ES-1B activities had a similar pH and temperature dependency and similar thermal stability profile. 2.
Jose V Montoya G et al.
Experimental neurology, 220(2), 303-315 (2009-09-15)
Embryonic spinal cord motor neurons (MNs) can be maintained in vitro for weeks with a cocktail of trophic factors and muscle-derived factors under serum-containing conditions. Here we investigated the beneficial effects of muscle-derived factors in the form of muscle-conditioned medium
Luke H Chamberlain
FEBS letters, 559(1-3), 1-5 (2004-02-28)
The relative insolubility of lipid rafts in cold non-ionic detergents is the most widely used method to purify these fascinating membrane domains from intact cells or membranes. Most of what we know about lipid raft function has been derived from
Xian-Man Zhang et al.
Chemistry and physics of lipids, 127(1), 113-120 (2004-01-07)
A rapid and simple approach using 1H NMR was developed for determination of liposomal encapsulation efficiency without the need for physical separation of entrapped and non-entrapped marker. Measurements were made using a marker (homocarnosine) with a pH-sensitive 1H chemical shift
Mammalian long-chain acyl-CoA synthetases.
Soupene E, Kuypers FA.
Exp. Biol. Med, 233, 507-521 (2008)
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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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