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Merck

MAK120

Sigma-Aldrich

脲酶活性检测试剂盒

sufficient for 100 colorimetric tests

别名:

Urea Amidohydrolase Activity Assay Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric tests

應用

environmental

檢測方法

colorimetric

相關疾病

gastrointestinal diseases

儲存溫度

2-8°C

一般說明

脲酶是一种催化尿素水解成二氧化碳和氨的酶。 脲酶存在于细菌、酵母和某些高等植物物种中。脲酶活性存在于环境和粪便样品中。

應用

脲酶活性测定试剂盒已用于测量脲酶活性。

特點和優勢

兼容高通量的操作系统。可在比色皿中使用。

適合性

适用于检测生物样品及环境样品中的脲酶活性。

原則

在该测定中,脲酶催化尿素的水解,导致氨的产生。 通过 Berthelot 方法测定氨,导致在 670nm 处测量比色产物,与样品中存在的脲酶活性成比例。一个单位的脲酶是在 pH 7.0 下,每分钟催化形成 1.0 μmole 氨的酶量。

訊號詞

Danger

危險分類

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

安全危害

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Chelsea M Heveran et al.
Scientific reports, 9(1), 14721-14721 (2019-10-13)
We demonstrate for the first time that the morphology and nanomechanical properties of calcium carbonate (CaCO3) can be tailored by modulating the precipitation kinetics of ureolytic microorganisms through genetic engineering. Many engineering applications employ microorganisms to produce CaCO3. However, control
Ureases I. Functional, catalytic and kinetic properties: A review
Barbara K.
Journal of Molecular Catalysis. B, Enzymatic, 59(1-3), 9-21 (2009)
Satya P G
Hydroxamic Acids: A Unique Family of Chemicals with Multiple Biological Activities (2013)
James M Wiebler et al.
Proceedings. Biological sciences, 285(1878) (2018-05-04)
Gut bacteria that produce urease, the enzyme hydrolysing urea, contribute to nitrogen balance in diverse vertebrates, although the presence of this system of urea-nitrogen recycling in Amphibia is as yet unknown. Our studies of the wood frog (Rana sylvatica), a
Bhawna Uprety et al.
Pharmaceutics, 14(1) (2022-01-22)
Tumour cells maintain a local hypoxic and acidic microenvironment which plays a crucial role in cancer progression and drug resistance. Urease is a metallohydrolases that catalyses the hydrolysis of urea into ammonia and carbon dioxide, causing an abrupt increase of

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