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應用
TMB是辣根过氧化物酶的底物。其可形成可溶性蓝色终产物，并可在370nm或655nm下读数。可以用0.5M H2SO4停止TMB反应（产生黄色溶液），并在450nm处读取数值。推荐用于ELISA（微孔）分析，不建议用于膜应用。
通过将测量的底物吸收值超过吸收最大值，3,3′,5,5′-四甲基联苯胺已在研究中用于证明，任何免疫酶测定的测量范围都可以被显著延长，而又不损害测定特征。3,3′,5,5′-四甲基联苯胺也已用于研究中，以了解具有过氧化物酶样活性的CuS凹面上层结构的形成。
通过将测量的底物吸收值超过吸收最大值，3,3′,5,5′-四甲基联苯胺已在研究中用于证明，任何免疫酶测定的测量范围都可以被显著延长，而又不损害测定特征。3,3′,5,5′-四甲基联苯胺也已用于研究中，以了解具有过氧化物酶样活性的CuS凹面上层结构的形成。
注意
该底物对光非常敏感，应避免阳光或紫外光源直射。
外觀
即用型。
訊號詞
Danger
危險分類
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1
標靶器官
Eyes,Central nervous system
水污染物質分類（WGK）
WGK 2
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商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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