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T4444

3, 3′,5 ,5′-Tetramethylbenzidine

Name: 3, 3′,5 ,5′-Tetramethylbenzidine
Brand: SIGMA

peroxidase substrate, chromogenic, aqueous solution

别名:

TMB

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About This Item

CAS号:

54827-17-7

MDL號碼:

MFCD00007748

分類程式碼代碼:

12352204

PubChem物質ID:

24900209

NACRES:

NA.83

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product name

3,3′,5,5′-四甲基联苯胺液体底物，超灵敏，用于 ELISA, ready to use solution

形狀

aqueous solution

運輸包裝

wet ice

儲存溫度

2-8°C

SMILES 字串

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI 密鑰

UAIUNKRWKOVEES-UHFFFAOYSA-N

一般說明

本品为包含3,3′,5,5′-四甲基联苯胺（TMB）的弱酸性缓冲液。底物以单组分即用型溶液提供。速率动力学约比传统TMB配方快40％。不推荐用于需要沉淀反应产物的膜或免疫组化应用。

應用

的3,3′,5,5′-四甲基联苯胺是超灵敏的ELISA液体底物，可用以下应用的作过氧化物酶底物：

通过转染子测定合成的透明质酸和HAS2（透明质酸合成酶）表达
测定结合的α-溶血素毒素的水平
结合IgG成像

3,3′,5,5′-四甲基联苯胺（TMB）是适用于ELISA程序的过氧化物酶底物。底物产生可溶的浅蓝色最终产物，并且可以在370或655nm下通过分光光度计读取。可以用2M H₂SO₄停止TMB反应（产生黄色溶液），并在450nm处读取数值。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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