SRP3190

Betacellulin from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 别名: BTC
• 分類程式碼代碼: 12352202
• NACRES: NA.32

属性

• 生物源: mouse
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形狀: lyophilized
• 效力: ≤0.01 ng/mL ED₅₀
• 分子量: 9.0 kDa
• 包裝: pkg of 20 μg
• 技術: cell culture | mammalian: suitable
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white to off-white
• UniProt登錄號: Q05928
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: mouse ... BTC(12223)

说明

一般說明

Betacellulin is a member of the EGF family of growth factors and is produced by an expression of a DNA sequence encoding the mature 80 amino acid residue polypeptide. The protein is expressed in the small intestine and pancreas. It is initially synthesized as a glycosylated 32.0kDa transmembrane precursor protein, processed by proteolytic cleavage to produce the mature sequence.

應用

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

生化／生理作用

Betacellulin and the other members of the epidermal growth factor (EGF) family of growth factors bind with high affinity to both EGFR and to erb-b2 receptor tyrosine kinase 4 (erbB-4). Overexpression of betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

注意

This product should be stored at -2-8°C. For longer-term storage, it is recommended to dilute in a buffer containing a carrier protein, for example, 0.1% BSA, and to store the solution frozen in working aliquots at 20°C or -80°C.

序列

DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY

準備報告

This product was lyophilized with no additives. It should be reconstituted by adding to water at a concentration of 0.1-1.0 mg/mL. The vial should be centrifuged prior to opening and the solution should not be vortexed.

分析報告

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC₅₀ is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable