SAB4200646

Monoclonal Anti-ALIX/PDCD6IP antibody produced in mouse

clone ALIX-1, purified from hybridoma cell culture

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: ALIX-1, monoclonal
• 形狀: buffered aqueous solution
• 物種活性: human
• 濃度: ~1 mg/mL
• 技術: flow cytometry: 5-10 μg/test using using human MOLT4 cells; immunoblotting: 2-5 μg/mL using using whole extracts of human MOLT4 cells.; immunofluorescence: 10-20 μg/mL using using human HeLa cells; immunoprecipitation (IP): 10-20 μg/mL using using lysates of human MOLT4 cells.
• 同型: IgG1
• UniProt登錄號: Q8WUM4
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PDCD6IP(10015)

说明

一般說明

Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP).

免疫原

peptide corresponding to an internal region of human ALIX/ PDCD6IP

應用

Monoclonal Anti-ALIX/PDCD6IP antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence
• flow cytometry

生化／生理作用

ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable