SAB4200485
Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-H3.4, Anti-H3/g, Anti-H3FT, Anti-H3K27ac, Anti-H3t, Anti-HIST3H3, Anti-Hist1h2ai, Anti-Hist1h2ail, Anti-Hist1h3a, Anti-Hist1h3g, Anti-Hist1h3h, Anti-Hist1h3i
登录查看公司和协议定价
所有图片(4)
About This Item
分類程式碼代碼:
12352203
NACRES:
NA.41
推荐产品
生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~15 kDa
物種活性
human, rat, mouse
濃度
~1.0 mg/mL
技術
immunohistochemistry: 10-20 μg/mL
indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells
western blot: 1-2 μg/mL using extracts of NIH3T3 cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
acetylation (Lys27)
基因資訊
human ... HIST3H3(8290)
一般說明
The HIST3H3 gene is mapped on the human chromosome at 1q42.13. Histones are proteins that form the nucleosome along with DNA. A nucleosome consists of core histone octamers made of two heterodimers of H2A and H2B along with two heterodimers of H3 and H4. This octamer is bound by the DNA and H1 protein.
特異性
Anti-acetyl-Histone H3 (Ac-Lys27) specifically recognizes human and mouse (Ac-Lys27) Histone H3 (H3K27Ac).
免疫原
synthetic peptide containing acetylated Lys27 of human histone H3, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H3
應用
Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit may be used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化／生理作用
Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination. Active chromatin is highly correlated with the hyperacetylation of histone H3 at the N-terminal tail. Histone H3 can be reversibly acetylated at Lys residues 9, 14, 18, 23, 27, 36 and 56 by a family of enzymes known as histone acetyltransferases (HATs). Histone H3 can be modified at Lys27 either by methylation or acetylation. Histones are thought to play an important role in development and cancer.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
儲存和穩定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Minghua Li et al.
Cell biology international, 45(6), 1278-1287 (2021-02-10)
One hallmark of renal cell carcinoma (RCC) is metabolic reprogramming, which involves elevation of glycolysis and upregulation of lipid metabolism. However, the mechanism of metabolic reprogramming is incompletely understood. Monocarboxylate transporter 1 (MCT1) promotes transport for lactate and pyruvate, which
Emanuely Silva Chrun et al.
Pathology, research and practice, 213(11), 1329-1339 (2017-09-09)
Among the epigenetic changes, histone acetylation has been recognized as a fundamental process that strongly affects gene expression regulation. Disrupt of this phenomenon has been linked to carcinogenesis. In this review, we analysed studies reporting the process of histone modification
Thomas H A Ederveen et al.
Biochimica et biophysica acta, 1809(10), 577-586 (2011-07-26)
Histones are highly basic, relatively small proteins that complex with DNA to form higher order structures that underlie chromosome topology. Of the four core histones H2A, H2B, H3 and H4, it is H3 that is most heavily modified at the
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门